NAME _______________ DATE ; ____________. 2012 Lab Instructor's Name; ___________________ Module #4 Lab Worksheet 1. Indicate bacterial growth in the following conditions with + or - Aerobic Anaerobic a) Obligate aerobe sample b) Facultative anaerobe sample c) Obligate anaerobe sample 2. How are obligate anaerobes grown in suspension or in agar plates are cultured in the laboratory? a). Liquid culture b). Agar plates 3. Bromcresol purple sugar fermentation tubes are used to determine if a given bacterium ferments a specific sugar (e.g., glucose or lactose). How do you recognize the formation of a) Acid b) Gas4. Discuss the role of sugar fermentation products in the etiology of dental caries (as indicated in the lecture (be brief). 5. Explain 2 reasons why drinking soda pops increase risk of dental caries? a) b) 6. Explain the procedure (culture medium and color reaction) employed to determine bacterial production of hydrogen sulfide (H2S). 7. What kind of bacterial enzyme causes gelatin liquefaction? Is this an intracellular or an extracellular enzyme? 8. What is a Gas-Pak and how is it used? 9. Do different bacteria vary in their ability to ferment sugars, produce H2S, and liquefy gelatin? How do we use this information to identify bacteria? 10. Describe a flow
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