NAME DATE:___________________________ Lab Instructor's Name;_____________________________ Module #16 Lab worksheet 1. What is the effect of boiling on; Gram (+) bacteria? Gram (-) bacteria/ Gram (+) endospore-forming rods? 2. What is the effect of autoclaving on; Gram (+) bacteria? Gram (-) bacteria/ Gram (+) endospore-forming rods? 3. What are appropriate conditions for autoclaving? 4. Do E. coli and B. subtilis react the same way to autoclaving and boiling? Why? 5. Sketch the appearance of B. subtilis containing endospores and vegetative cells. 6. What effect does sporulation have on bacterial survival? 7. Describe a common disease that is caused by bacteria that produce endospore 8. Describe the difference between sterilization and disinfection. 9. Indicate properties of these disinfectants? Zephiran: Merthiolate:10. Why is there growth in the diluted tube with a static disinfectant? 11. Why is there no growth in the diluted tube with the cidal disinfectant? 12. Why would a bacteriostatic agent be useful in aiding the healing of a skin wound in humans? Why are bactericidal agents not always used? 13. Is time a factor in using disinfectants? In sterilization procedures? 14. Is this a true statement: "There is one best disinfectant for all purposes"? Explain your answer. 15. Why presence of blood reduces the effectiveness of some disinfectants. 16. Assume one of your coworkers slips on the hall floor and drops a tube of blood. Describe how you would clean the spill so that you are protected and anyone who walks by will be protected while you
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