SC BIOL 302 - Final Exam Study Guide (4 pages)

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Final Exam Study Guide



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Final Exam Study Guide

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Pages:
4
Type:
Study Guide
School:
University Of South Carolina-Columbia
Course:
Biol 302 - Cell & Molecular Biology
Edition:
1

Unformatted text preview:

BIOL 302 1nd Edition Exam 3 Study Guide Lectures 14 18 Lecture 14 Role of Chromatin histone modifications in transcription You cannot get DNA because there is limited access to the nucleosomes and chromatin due to it repressing transcription DNA cannot reach it SWI SWF have enzymatic activities that modify to allow recognition by other factions and access to DNA sequences there are 10 proteins in a complex Hydrolysis of ATP is used to change the organization of the nucleosomes Histones are chemically modified to allow recognition Histone acetyl tranferase HAT and Histone deacetyl transferase DHAT HAT adds an acetyl group that tends to make it active while DHAT removes it and inactivates it The acetyl group makes it negatively charged due to the histones losing their grip on the DNA and the genes are turned on Carboxyl terminal with the NH terminal are with all the positive charges to interact with the negative at neutral pH Transcription Termination Bacteria cells have a termination signal in termination while eukaryotic cells do not E Coli can end the process by using the termination signal in the DNA that s transmitted in the DNA by RNA it releases polymerase and mRNA from template ACTIVE Properties termination signal is in DNA and signal transmitted by RNA transcript 3 prime end where there are a bunch of U s in the sequence that undergoes internal base pairing E Coli Termination Sequence it makes a stem loop structure with A and U s at the end RNA polymerase makes this transcript and synthesizes and forms a double stranded structure It should be G C rich stem loop of 8 or so nucleotides which makes it stable and all the U s have to be at the bottom of the RNA Eukaryotic Termination transcription occurs and makes RNA molecule not functional messenger RNA Sequence is required and presence in Eukaryotic cells mRNA at 20 10pt 3 prime CF cleavage factors CPSF cleavage and polyadenylation specificity factor Polydenylation signals a reaction that cuts 3 end but keeps



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