SC BIOL 302 - Splicing (2 pages)

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Description of splicing

Lecture number:
Lecture Note
University Of South Carolina-Columbia
Biol 302 - Cell & Molecular Biology
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BIOL 303 1nd Edition Lecture 16 Outline of Last Lecture I Transcription a Role of chromatin II Termination a Capping b Poly A tail III Splicing Outline of Current Lecture I RNA Splicing Current Lecture I RNA Splicing a There 3 different components i Specific sequences on the RNA that extinguishes introns and exons 1 Present at every intron exon and is highly conserved 2 CAG ending of an exon GU starts an intron CAG ends an intron and demarks the sequence 3 2 ways to recognize a Protein b By other nucleotides base pairing ii Splicisomes recognizes and identifies the sequences 1 Large multi protien RNA sequence 2 5 different complex each has 10 15 different proteins contains 1 small RNA U1 U2 3 Small RNA are required for splicing because it bonds to exons and introns 4 The U1 RNA base pairs with the sequences with the adenine group in the intron A 5 Curved with protein 2 OH of adenine does the cutting of the phoshposidester bond and the phosphate of the guanine gets a bond with the adenine 6 U5 cuts between the end of intron and beginning of exon and the larat the closed loop being released and destroyed These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II iii Auto hydrolysis RNA is active self contained Splicing defects a Destroys splice set instead of CAG it s CAU and it doesn t recognize as an intron and completely skips it because it thinks it s at the beginning of an intron b Alternative Splicing primary transcript has too many exons that are used at one time i Causes the protein to be different ii Ex fibroblast skips the exons 7 and 10 1 Muscle skips the 7 and 11 this results in 2 different proteins multiple genes c Wrap p53 primary transcript with multiple splicing product spliciosomes must vary and alter and has to be very coordinated d Protein Domains exons within you get entire domain i Enzymes of protease with certain conditions ways genes are made up of exons and would be able to recombine into new activity

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