SC BIOL 302 - Termination (2 pages)

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Termination



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Termination

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Describes mostly the difference of Transcription


Lecture number:
15
Pages:
2
Type:
Lecture Note
School:
University Of South Carolina-Columbia
Course:
Biol 302 - Cell & Molecular Biology
Edition:
1
Unformatted text preview:

BIOL 303 1nd Edition Lecture 15 Outline of Current Lecture I Transcription a Role of chromatin II Termination a Capping b Poly A tail III Splicing Current Lecture I Transcription a Simplification there is no DNA structure shown but the gene that is in there b For the signal you can t get DNA because there is limited access to nucleosomes and chromatin which represses transcription c SWI SWF have enzymatic activities that modify to allow recognition by other factions and access to DNA sequences d Histone acetyl transferase HAT adds the acetyl group e Histone de acteyl transferase HDAT which removes the acetyl group i C terminal NH terminal with all positive charges to interact with the negative at neutral pH charged ii The acetyl group makes it negatively charged due to the histones losing their group their grip on the DNA genes are on f HDAT inactive HAT active i Promoters specific interacts with tafs 1 Ex SP1 with taf 250 when the transcription is building up it brings the taf250 in the DNA complex and acts as the recruiter to the taf250 and starts to undo the chromatin 2 Happens on different histones at different times respecting to the gene expression g H3 super complicated i All modifications can happen in different paths with different combinations the Histone code that signals certain codes with certain genes that determine if they re going to turn on off and carry out the same actions h 30nm Fiber somewhere there has to be a gene that turns on These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II III i Acts as a substrate pioneer factor replaces A and starts to come apart and remodels the chromatin beads on a string 1 Ex Turning on insulin we don t know where the location is but modification on the genes to expose where they are ii Bre1 factor that locates where the gene is iii Pioneer replaces histones H1 and starts to come apart ex A1 Termination a In bacteria cells termination sequences stops transcription and releases polymerase and mRNA from the template but in prokaryotic cells they do not b E Coli can end the process with termination signal is in the DNA transmitted by RNA when in it becomes active i Bunches of U s ii Undergoes and makes a stem loop c RNA makes the transcription it immediately shapes up and makes a double strand d Binds to NUSA RNA strand inhibits the polymerase from transcribing until it reaches the last U e What s required for termination 2 i Has to be a GC rich stem loop within 8 nucleotides because it makes it stable ii All the U s have to be at the end unstable this helps recognize the structure f Eukaryotic Termination i There s not really any terminating going on ii Sequence is required and presence in Euk mRNA at the 20 10 point 3 end polyadenylation signals a reactions that cuts 3 end but keeps the polymerase going also the RNA falls off iii Primary transcript has to be modified in 3 ways 5 cap at AAA and splicing Splicing the introns needed to be removed because the exons are needed to be together a 5 cap happens in every message mRNA soon after initiation but before termination b A G are the start sites in most genes 5 end has the triphosphate and guanine with methyl group which is added on the 5 carbon to the 5 end


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