BCHM 4116: General Biochemistry
School: Virginia Polytechnic Institute and State University (Virginia Tech )
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From: GradeBuddy Pages: 6Using clones for experiments and finding the right match using primers and probes. These methods are called southern/northern blots, and library screening. -
From: GradeBuddy Pages: 7These notes go over the main ways that labs clone genetic material, and their advantages and disadvantages. Additionally, the creation of a genomic library is discussed. -
From: GradeBuddy Pages: 5What is cloning, and how are some ways to increase update of inserts in vector. The traditional way to do cloning. -
From: GradeBuddy Pages: 3In order to create DNA fragments, DNA needs to be cut using physical, chemical or enzymatic methods. These fragments can then be annealed using ligase. -
From: GradeBuddy Pages: 2The genome is complex, and large, and there are many ways of making a longer genome that also lend in creating diversity. -
From: GradeBuddy Pages: 3Look at how the building blocks of genetic material build up from nucleotides, to genes, to the entire genome, and does the size of a genome correlate with biological complexity? -
From: GradeBuddy Pages: 5Effects of different agents on DNA, and the presence of differing tertiary structures in DNA -
From: GradeBuddy Pages: 3The parts that make up a DNA and RNA strand, and the bonds that hold them together. Stability of DNA vs RNA. -
From: GradeBuddy Pages: 2Lecture 1 Notes: Goes over the central dogma, introduces the idea of RNA World hypothesis, and new developments that challenge the central dogma.