UT Knoxville BIOL 140 - Lab Communication 1 (4 pages)

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Lab Communication 1



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Lab Communication 1

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Pages:
4
School:
University of Tennessee
Course:
Biol 140 - Organization/Function of Cell
Organization/Function of Cell Documents

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Payal Patel Biology 140 Section 16 Amanda McClelland Lab Communication 1 The most effective antibiotic on strain C E Coli Introduction I learned from the readings that antibiotic resistance is not something that the bacteria consciously do Resistance is created through mutations and genetic differences that are replicated and produced from the initial surviving bacteria Many strains of antibiotic resistant Tuberculosis are present today because people who start the antibiotic regimen many times do not finish them because of its high cost and time commitment Because of this a few of the bacteria that survive the beginning rounds of antibiotics because of resistance replicate and create new colonies that are harder to combat than the earlier strain Tuberculosis is still prevalent today and not just in the poorer regions of the world but also in the United States This is why it is important for us to analyze resistance in bacteria Barry and Cheung 62 64 To experiment and observe resistance bacteria have to antibiotics we experimented with a nonpathogenic E Coli strain C We hypothesized that if strain C is a wild type E Coli without transferases it will be susceptible to the aminoglycosides Tubramycin Kanamycin and Amikacin because they disrupt protein synthesis We predicted that if strain C is a wild type strain the aminoglycosides will be effective Methods In our group the materials we used were 4 agar plates sharpie tape strain C E Coli swabs rubbing alcohol forceps antibiotic disks and blank disks To begin we split each plate into an equal four parts with a sharpie and labeled what disk we would place on each section one being a blank disk control We then swabbed a lawn of strain C E Coli onto each plate In the middle of each section a disk was placed In the first set of plates 2 replicates ciprofloxacin Amikacin and kanamycin antibiotic disks were placed in the middle of sections one on each section On the fourth section a blank control disk was placed On the



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