UT Knoxville BIOL 140 - Lab Communication 3 (6 pages)

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Payal Patel Lab Communication 3 Section 16 Group 4 Yeast Biofilm and It s Growth Medium Introduction Biofilms are groups of an organism or many organisms that stick to organic and nonorganic sources It has been shown that although these cells are not of the same organism or species they communicate with one another as a multi celled organism would do They grow share resources and often have set functions for the better of the biofilm Yeast is used for the production of alcohol such as wine This is because the yeast ferments with a carbon source to produce an alcohol byproduct Hall Stoodley Costerton Stoodley From the background information that was collected it was hypothesized that lactose would be a better growth medium for the yeast than glycerol because it goes through the carbohydrate metabolism rather than an alternate pathway such as lipid biosynthesis So the prediction was that lactose would have more overall growth of the yeast than glycerol Methods In the experiment the control was the dextrose plate The variables manipulated were the carbon source which was the independent variable and the amount of growth in each of the four strands of yeast used the dependent variable There were 2 replicate plates of lactose and 2 replicate plates of glycerol with only one control dextrose plate All were inoculated in the same fashion with the same strains From the initial observations there was the observation that 0 3 agar plates had more growth of yeast than the 0 2 agar plates and the sacc wild type had the most growth overall 5 measurements were taken of each strain on the 0 3 agar plates pH of agar on the edge of the plate pH on the edge of the biofilm pH in the center of the biofilm diameter of the biofilm in millimeters and the adhesion overlay test The plates were inoculated and incubated at 37 degrees Celsius for seven days then measured To make sure there were no discrepancies the diameter of the biofilm was taken in two perpendicular directions and averaged