UT Knoxville BIOL 140 - Lab Communication 2 (3 pages)

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Lab Communication 2



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Lab Communication 2

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Pages:
3
School:
University of Tennessee
Course:
Biol 140 - Organization/Function of Cell
Organization/Function of Cell Documents

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Payal Patel Lab Communication 2 Section 16 Group 4 Which carbon source promotes the most bacterial growth Introduction The Krebs Cycle is how E coli bacteria processes the carbon sources it obtains It is a part of aerobic respiration The goal at the end of the cycle is to produce ATP which is used for energy by the cell The beginning processes of the Krebs Cycle uses glucose which is further broken down into other carbon sources The other carbon sources further through the cycle can also be acquired by the cell to be used to process into ATP Schussler Hudson Rowe Lemieux Naswa 2012 After reading the article and relating it back to lecture it was observed that glucose was more versatile and was the very beginning product that could be used in the Krebs Cycle From the background information we hypothesized that glucose would have more bacterial growth than glycerol because it can be broken down into more usable forms We predicted that glucose would be more efficient in causing growth than glycerol We think this will be the result because glucose is involved in the very beginning of the Krebs Cycle and this would be efficient because it can be broken down and adjusted to the current needs of the bacteria Methods In our experiment our controls were our blanks one with glycerol and another with no carbon source The variable manipulated the independent variable in other words was the carbon source that was used Two carbon sources were used under the same conditions glycerol and glucose The independent variable caused our amount of absorbance or rate of optical density to vary and this is our dependent variable In this experiment the growth of bacteria was tested which was measured by optical density with a spectrophotometer For the glucose there were no replicates When testing glycerol there were two replicates for every time interval for glycerol and the no carbon blank To set up the experiment first blanks were made by putting 4 mL of our no carbon source glycerol and



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