Quiz 1: Molecular techniques (10 points) (Get zero point if failed to check all correct answers)*Please review lecture 5B on molecular techniques before taking Quiz 1*Multiple Choices. Check all that apply*Can be attempted twice before Oct. 2 (test ending Oct. 2 11:59 PM)* You have 20 minutes to work on this Quiz once you start1. You can detect a single molecule of DNA from fossils usinga. Northern blotb. Southern blotc. PCRd. Sanger sequencing2. In gel electrophoresis, DNA fragment moves towarda. Positively charged poleb. Negatively charged pole3. The faster a DNA fragment moves through the gel, the _________ the fragment is.a. Biggerb. Smaller4. During Sanger DNA sequencing, the elongating chain (the nascent DNA chain) terminates after incorporatinga. Dideoxyribonucleotide b. Deoxyribonucleotide c. Ribonucleotide 5. In Southern or Northern blot, a probe is required to detect corresponding target molecule. The process of the probe paring with its complementary target sequences is called:a. Probe labelingb. Transferc. Blottingd. Hybridization6. PCR consists of many cycles of three successive steps and requires following a. NTPb. Primersc. Taq polymersesd. All of above7. when following restriction enzymes were used to digest the human genomic DNA. Which enzyme yields the least number of fragments?a. EcoR1 (6 cutter) recognizes and cuts a sequence specified by a 6 nucleotide sequence.b. Not I (8 cutter)c. Sau3AI (4 cutter)8. cDNA stands for complementary DNA. It is made with a. Taq polymeraseb. Reverse transcriptasec. mRNA as a templated. Genomic DNA as a template9. PCR has many advantages over Southern blot.a. Requires primersb. Fasterc. Cheaperd. Safere. Can amplify minute amount of templatef. All of above10. In molecular cloning, a human gene can be cloned into a plasmid and then propagated inside the host bacteria. If the cloned human gene contains an intron, will the host bacteria be able to replicate the cloned human DNA?a. Yesb.
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