11. Expression Library2. HybridizationSouthern and Northern blotsColony hybridization3. PCR (Polymerase Chain Reaction)4. DNA sequencingRead 58-62; 64-68; 241-245Fig. 2.14-16; 2.19-20; 6.23-26Molecular Techniques II2Fig. 9.12Expression Library3Hybridization-probes4Fig. 9.5b4Southern Blot5Fig. 9.15b6Fig. 9.15c7Fig. 9.15d89Fig. 9.13Colony hybridization10Colony hybridization11Fig. 9.16The Polymerase Chain Reaction (PCR)1112Fig. 9.16c13Fig. 9.16d1414PCR movie15Generate & align large BAC or P1 clonesFragment and sequence a subset of clonesHierarchical sequencingFragment and sequence entire genomeShotgun sequencingAdapted from Fig. 2.7 Gibson and MuseDNA sequencing16Fig. 9.17aSanger Sequencing(chain termination with a specific ddNTP (dideoxynucleotides)17Fig. 9.17b18Fig. 9.17c1819Fig. 9.1819202021Personal Genome Project (PGP) Goal: sequencing full genome of individuals at $1000Human Genome Project (HGP) (total 3 billion $) :Motivated 100X reduction in cost (10 $ per base to 10 base per 1$.)Personal Genome Project(PGP: has motivated thedevelopment of Ultra-Low-Cost-Sequencing
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