Quiz 3: Markers and Mapping (10 points)*Please review lecture 6-8 before taking this quiz*Multiple Choices. Only one correct answer per question*Please have pencil and scratch paper ready*Can be attempted twice before Oct. 2 (test ending Oct. 2 11:59 PM)* You have 30 minutes to work on this quiz once you start1. If a SNP could be distinguished by restriction enzyme digestion, it is also calleda. Microsatellite markerb. Minisatellite markerc. Indel (insertion/deletion)d. Restriction fragment length polymorphism (RFLP)2. DNA fingerprint was based which of following DNA polymorphisms?a. Microsatellite markerb. Minisatellite markerc. Indel (insertion/deletion)d. Restriction enzyme polymorphism (RFLP)3. Trinucleotide repeats underlie many human genetic diseases. They also serve as good molecular markers due to fast expansion or contraction of the number of repeats in the human population. What type of polymorphism is trinucleotide repeat?a. Microsatellite markerb. Minisatellite markerc. Indel (insertion/deletion)d. Restriction enzyme polymorphism (RFLP)4. Which method should one use to assay trinucleotide repeat polymorphisms?a. Southern blot combined with probe hybridizationb. Restriction enzyme digest followed by electrophoresisc. PCR followed by gel electrophoresisd. Any of the above methods5. Below is an electrophoresis image of PCR amplified fragments from 11 individuals. Individuals with the M disease allele exhibit the top (bigger size) band. Individuals with the wild type A allele exhibit the lower (smaller size) band. If M allele is recessive, how many individuals willdevelop M disease? _____4___6. (Continue from question 5) How many individuals are carriers of the disease allele M? _____2______7. (Continue from question 5), How many individuals are completely wild type in phenotype and genotype? _____5_____8. The AA or Aa genotypes show Blue kernel while the aa genotype shows yellow kernel. A second locus W (WW or Ww) causes smooth kernel, while the ww kernel is wrinkled. A smooth blue kernel plant is crossed with a plant with yellow and wrinkled kernel. The F1 progeny consists of following phenotypic categories: 900 blue smooth, 100 blue wrinkled, 102 yellow smooth, and 898 yellow wrinkled. Are the A and W loci linked? a. Yesb. Noc. Not able to determine9. (Continue from question 8) If A and W are linked, how far apart are they from each other. a. 0.1 cMb. 1cMc. 10cM10. What is the genotype of the Blue smooth parent?a. Aa Ww b. AA WWc. Aa WWd. AA
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