E. Suppression Intrgenic suppressorsExtragenic suppressorsAllele-specific suppressionSuppressors are defined classically as mutations that correctthe phenotypic defects of another mutation without restoringits wild-type sequence. Suppressors may be intragenic(affecting the same gene) or they may be extragenic(affecting a different gene). Extragenic suppressors areparticularly useful during genetic analyses, because theyoften identify additional components of a biological systemor process.Allele-specific suppressionIn c. elegans, eight suppressors encode identical tRNAsin which a single C→T substitution changes theanticodon of a tRNATrp gene from 5′-CCA-3′to 5′-CUA-3′. The anticodon change thus allows mutanttRNAs to read the amber codon UAG.Allele-specific information suppressorsIntragenic suppressorsMutant1Mutant1 +
View Full Document
Unlocking...