UMD BSCI 410 - Lecture 6: Molecular Techniques - D2620288

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UMD BSCI 410 - Lecture 6: Molecular Techniques

School name University of Maryland, College Park

Course Bsci 410- Molecular Genetics

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1Lecture 6: Molecular Techniques1. Restriction Mapping2. Molecular cloning (recombinant DNA)Inserts and vectorsRestriction enzymes and ligaseFig. 2.9-11; 12.2-10Table 2.3Read p53-58; p502-5122Fig. 9.2Fig. 9..2234Fig. 9.65Fig. 9.5b56Gel electrophoresis672. Molecular CloningInserts and vectors8Restriction Digestion89Fig. 9.710Fig. 9.81011Fig. 9.91112Genomic LibraryHuman: 3,000,000 kb/haploid genome150 kb per insert--20,000 clones equals a genome10kb per insert--300,000 clones (genome equivalent)cDNA libraryExpression Libraries13Fig. 9.1014Fig.

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