Quiz%8:%More%Functional%Genomics.%This%quiz%covers%the%following:%%%i. Lecture%14%ii. Reading%material%that%was%assigned%for%the%Week%of%Oct%29%(see%Oct%29%Blackboard%announcement)%%iii. The%2%short%recorded%lectures%on%targeted%gene%replacement%(see%Oct%30%Blackboard%announcement).%%%%%%%Worth%20%points%total.%%(1%pt%each)%%1.%Suppose%you%are%interested%in%finding%genes%involved%in%human%brain%function%and%using%a%microarray,%you%identify%45%genes%that%are%highly%expressed%in%the%brain%and%weakly%expressed%in%other%organs%of%the%body.%%Next%you%will%study%the%functions%of%these%genes.%%Which%genetics%approach%does%this%represent?%a. Forward%genetics%b. Reverse%genetics%c. Both%d. None%%2.%To%study%the%functions%of%the%genes%above,%you%wil l %need%to%use%functional%genomics%approaches.%%Such%approaches%aim%to%describe/understand%genes%at%a%dynamic%functional%level%(e.g.,%gene%activity),%beyond%knowing%only%the%DNA%sequence.%%Which%of%the%following%is%NOT%an%experimental%strategy%in%functional%genomics?%%%% a.% approaches%that%describe%or%identify%gene%expression%patterns%% b.% approaches%that%describe%or%identify%protein%expression%patterns%% c.% approaches%that%describe%or%identify%proteinTprotein%interactions%% d.% approaches%that%describe%or%identify%mutant%phenotypes%% e.% none%of%the%above%%3.%One%of%the%approaches%in%your%molecular%genetic%toolkit%is%the%yeast%twoThybrid%assay.%%The%yeast%twoThybrid%assay%is:%%%a. a%gene%knockout%method%b. not%applicable%to%human%brain%genes%c. a%method%for%assaying%protein?protein%interactions%d. a%method%for%examining%the%expression%of%a%gene%of%interest%e. a%method%for%examining%the%expression%of%a%protein%of%interest%%%True%or%False.%%%4.%%In%certain%organisms,%targeted%gene%replacement%is%a%powerful%approach%that%allows%one%to%disrupt%any%specific%gene%for%the%purpose%of%identifying%a%mutant%phenotype%for%the%gene.%%Mark%each%of%the%following%statements%as%either%TRUE%or%FALSE%regarding%targeted%gene%replacement:%a. It’s%a%method%for%assaying%proteinTprotein%interactions%%FALSE%b. It’s%based%on%homologous%recombination%%TRUE%c. It’s%a%standard%procedure%in%Drosophila,%mice%and%C.,elegans,,,FALSE%d. It%allows%you%to%determine%the%gene%expression%pattern%for%a%gene%of%interest%%FALSE%e. It%can%be%utilized%in%a%genomeTwide%mutant%screen,%primarily%in%yeast.%%%TRUE%%5.%Affinity%capture%or%CoTimmunoprecipitation%(coTIP)%is%a%method%for%identifying%proteins%that%interact%with%a%protein%of%interest.%%Mark%each%statement%regarding%this%method%as%either%TRUE%or%FALSE:%a. It%involves%a%yeast%cell%lysate%or%a%protein%extract%from%yeast.%%FALSE%b. It%involves%mass%spectrometry%to%identify%the%proteins%that%interact%with%the%protein%of%interest%%%TRUE%c. It%requires%an%antibody%that%recognizes%the%protein%of%interest.%%%TRUE%d. It%requires%antibodies%that%recognize%the%proteins%that%interact%with%the%protein%of%interest.%%%%FALSE%%6.%Mark%each%statement%as%TRUE%or%FALSE%regarding%the%yeast%twoThybrid%assay:%%a.% It%requires%three%plasmids%%%FALSE%b.% It%requires%an%antibody%%%%%%%%%FALSE%c.% It%takes%place%in%yeast%cells%%TRUE%%7.%Mark%each%statement%as%TRUE%or%FALSE%regarding%targeted%gene%replacement%in%mice:%%a.% It%requires%a%strategy%that%is%based%on%thymidine%kinase%(encoded%by%the%“tk”%gene%to%eliminate%cells%that%have%undergone%nonThomologous%recombination.%%TRUE%b.% You%don’t%want%the%embryonic%stem%(ES)%cells%that%end%up%carrying%the%neomycin%resistance%marker%gene.%%%%FALSE%c.% Involves%transfection%of%your%DNA%targeting%vector%into%embryonic%stem%(ES)%cells.%%TRUE%d.% The%targeted%ES%cells%are%injected%into%blastocysts%and%then%implanted%into%a%surrogate%mother%mouse.%%TRUE%e.%
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