IUB BIOL-L 211 - Translation II (4 pages)

Previewing page 1 of 4 page document View the full content.
View Full Document

Translation II

Previewing page 1 of actual document.

View the full content.
View Full Document
View Full Document

Translation II


Translation Elongation and Termination

Lecture number:
Lecture Note
Indiana University, Bloomington
Biol-L 211 - Molecular Biology
Documents in this Packet

Unformatted text preview:

BIOL L211 Lecture 32 Outline of Last Lecture I Recruiting Ribosome to mRNA transcript II Initiation of Prokaryotic Translation III Initiation of Eukaryotic Translation IV Eukaryotic Translation Outline of Current Lecture I Translation Elongation II Translation Termination Current Lecture Translation II I Translation Elongation A No significant difference that we will cover between eukaryotes and prokaryotes B Major Steps 1 Codon in A site determines which tRNA is recruited and loaded 2 Amino acids are linked by a peptide bond 3 Polypeptide from tRNA in P site shifts to the charged tRNA in the A site 4 Ribosome is transferred toward 3 end of mRNA a A new codon is then in the A site b Empty tRNA is ejected via the E site 5 Repeat Until stop codon C Elongation Factor EF Tu These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute 1 EF Tu elongation factor responsible for binding and bringing charged tRNAs to A site 2 EF Tu GTP ensures tRNA binds at appropriate time 3 GTP hydrolyzes when tRNA binds correct codon which causes the release of EF Tu GDP 4 Accommodation rotation of charged tRNA into the peptidyl transferase center D Peptide Bond Formation 1 Incorrect codon anticodon pairing causes a Reduced EF Tu GTPase activity b No accommodation 2 For Peptide Bond to form correctly charged tRNA must be in A site AND have gone through accommodation 3 Bond between amino acids is called a peptide bond and is formed through a dehydration reaction loss of water 4 rRNA in large subunit catalyzes peptide bond formation 5 Protein directionality N terminus to C terminus E Elongation Factor EF G 1 Translocation Movement of tRNA from A site to P site and movement of uncharged tRNA to the E site shift down one codon 2 After peptide chain is moved to A site tRNA tRNAs shift to hybrid state their 3 Hybrid State state in which 3 ends of the tRNAs are in new positions in the large ribosomal

View Full Document

Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...

Join to view Translation II and access 3M+ class-specific study document.

We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Translation II and access 3M+ class-specific study document.


By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?