IUB BIOL-L 211 - Translation II (4 pages)

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Translation II



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Translation II

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Translation Elongation and Termination


Lecture number:
32
Pages:
4
Type:
Lecture Note
School:
Indiana University, Bloomington
Course:
Biol-L 211 - Molecular Biology
Edition:
1
Documents in this Packet

Unformatted text preview:

BIOL L211 Lecture 32 Outline of Last Lecture I Recruiting Ribosome to mRNA transcript II Initiation of Prokaryotic Translation III Initiation of Eukaryotic Translation IV Eukaryotic Translation Outline of Current Lecture I Translation Elongation II Translation Termination Current Lecture Translation II I Translation Elongation A No significant difference that we will cover between eukaryotes and prokaryotes B Major Steps 1 Codon in A site determines which tRNA is recruited and loaded 2 Amino acids are linked by a peptide bond 3 Polypeptide from tRNA in P site shifts to the charged tRNA in the A site 4 Ribosome is transferred toward 3 end of mRNA a A new codon is then in the A site b Empty tRNA is ejected via the E site 5 Repeat Until stop codon C Elongation Factor EF Tu These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute 1 EF Tu elongation factor responsible for binding and bringing charged tRNAs to A site 2 EF Tu GTP ensures tRNA binds at appropriate time 3 GTP hydrolyzes when tRNA binds correct codon which causes the release of EF Tu GDP 4 Accommodation rotation of charged tRNA into the peptidyl transferase center D Peptide Bond Formation 1 Incorrect codon anticodon pairing causes a Reduced EF Tu GTPase activity b No accommodation 2 For Peptide Bond to form correctly charged tRNA must be in A site AND have gone through accommodation 3 Bond between amino acids is called a peptide bond and is formed through a dehydration reaction loss of water 4 rRNA in large subunit catalyzes peptide bond formation 5 Protein directionality N terminus to C terminus E Elongation Factor EF G 1 Translocation Movement of tRNA from A site to P site and movement of uncharged tRNA to the E site shift down one codon 2 After peptide chain is moved to A site tRNA tRNAs shift to hybrid state their 3 Hybrid State state in which 3 ends of the tRNAs are in new positions in the large ribosomal



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