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IUB BIOL-L 211 - New Life for Ancient DNA

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BIOL-L 211 1st Edition Lecture 1 Lecture Notes (Note: First day of lecture- minimal material covered)I. Molecular Biology in the NewsA. Article: "New Life for Ancient DNA" – Scientific American, August 20121. Genetic study performed with a preserved mammoth2. Purpose: to evaluate the influence of mammoths' genetic makeups on the ability of their hemoglobin to function despite cold temperaturesa. Hemoglobin (a protein) binds to oxygen in lungs and delivers it to tissues throughout the bodyb. Hemoglobin does not readily release oxygen in cold temperaturesc. Resulting question: How did mammoths adapt such that their hemoglobin was unimpaired by the cold temperatures?3. Procedure:a. Researchers sequenced the mammoth hemoglobin gene from preserved fragmentsb. Next, the sequenced gene was compared to the hemoglobin gene froma modern day elephantc. The mammoth gene differed from the elephant by three nucleotides4. Drawing a Conclusion:a. The new hemoglobin gene was introduced to E. Coli (which are excellent for rapidly producing copies of genes), and the E. Coli was then exposed to cold temperaturesb. The result confirmed that the E. Coli was better adapted to the cold environment with the new gene sequenceThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.c. Researchers were able to conclude that the mammoth's hemoglobin gene sequence did specifically code for a mechanism to allow hemoglobin to function despite cold


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