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IUB BIOL-L 211 - Gene Silencing

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BIOL-L211 Lecture 28 Outline of Last Lecture I. Drosophila Dscam GeneII. mRNA ExportIII. Regulatory RNAsIV. miRNA PathwayOutline of Current Lecture I. RecapII. Gene Silencing by miRNAs and siRNAsIII. ArticleCurrent LectureGene SilencingI. RecapA. siRNAs and mRNAs are processed by Dicer1. Cuts both into short dsRNA fragments B. siRNAs and miRNAs are bound by Argonaute1. Argonaute: central component of RISC (RNA-induced silencing complex)C. RNA-RNA base pairing directs siRNAs and miRNAs to target mRNAII. Gene Silencing by miRNAs and siRNAsA. Methods for Gene Repression by RISC1. Cleavage and digestion of mRNAThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.a. Requires a perfect match between the first 7 to 8 bases of miRNA and the mRNA2. Translation inhibitionb. Occurs with an imperfect match between miRNA sequence and mRNA3. Recruitment of chromatin-modifying enzymesIII. ArticleA. "Defeating Nature's Terrorists" –Scientific American, April 2014B. "FDA Oks siRNA Ebola Drug" –The Scientist, September 2014C. Current application of siRNAs:1. Silencing of Ebola genes (which aren't present in human DNA)a. Researchers encapsulated siRNAs in lipid nanoparticles (LNPs)2. High doses of injected LNPs have a high experimental success ratea. Product: TKM-Ebolai. Drug not FDA approved yet for use in humansii. Still, FDA permitting infected patients to receive TKM-Ebola


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