IUB BIOL-L 211 - Replication II (3 pages)

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Replication II



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Replication II

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Replication II


Lecture number:
9
Pages:
3
Type:
Lecture Note
School:
Indiana University, Bloomington
Course:
Biol-L 211 - Molecular Biology
Edition:
1
Documents in this Packet

Unformatted text preview:

BIOL L 211 Lecture 9 Outline of Last Lecture I Replication Hypotheses II Meselson Stahl Experiment III Methods and Minutia of DNA Replication Outline of Current Lecture I DNA Polymerase II Sliding Clamp Loader III Finishing Replication IV E Coli Holoenzyme and Replisome Current Lecture Replication II I DNA Polymerase A Processive Adds many dNTPs before leaving template strand 1 DNA can synthesize quickly 2 1000 dNTPs second B Exonuclease allows proofreading and removes improper base pairs 1 Only fixes most recent errors 2 Mispairing delays DNA synthesis 3 Causes the primer template junction to destabilize moving to the exonuclease active site C Sliding clamp donut shaped protein complex around DNA that prevents DNA polymerase from diffusing away These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute 1 Without sliding clamp DNA polymerase could only add 10 15 bases before falling off the template strand 2 Does NOT interact with primase II Sliding Clamp Loader Function A Loads the Sliding Clamps analogous to the claw from Toy Story B ATP binds to the clamp loader which binds and opens the sliding clamp C Sliding Clamp is placed at the primer template junction D ATP hydrolysis lets clamp loader go after which the sliding clamp closes spontaneously III Finishing Replication A RNase H endonuclease removes most of the RNA primer 1 Endonuclease enzyme that removes nucleotides internal to DNA B 5 Exonuclease removes nucleotides from the end of DNA C DNA Polymerase fills in the gap except for one little spot which is finished by DNA ligase D Linear Chromosome Problems 1 Lagging Strand DNA polymerase cannot fill in the end of chromosomes without primer which naturally leads to a gradually shrinking chromosome 2 Telomeres Ends of linear chromosomes 3 Telomerase solves the problem of the lagging strand by replicating ends of linear chromosomes both protein and RNA a Binds to the



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