IUB BIOL-L 211 - DNA and Transposons (3 pages)

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DNA and Transposons

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DNA and Transposons


DNA and Transposons

Lecture number:
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Indiana University, Bloomington
Biol-L 211 - Molecular Biology
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BIOL L 211 Lecture 5 Outline of Last Lecture I Bacteria II Bacteriophage III DNA Outline of Current Lecture I DNA Components II DNA Genetic Sequence III Transposons IV Pseudogenes Current Lecture DNA I DNA Components A Chromatin complex of DNA protein B Histones Proteins that compact eukaryotic chromosomes not present in prokaryotes C Nucleosome unit of DNA wrapped around histones D Diploid somatic cell 2 complete sets of chromosomes or Haploid gamete one set of unpaired chromosomes E Gene Density correlates to the amount of Junk DNA DNA that doesn t code for anything more complex organisms tend to have smaller gene density more junk DNA separates genes II DNA Genetic Sequence A Introns Non protein coding regions within genes note introns interruptions B Exons Coding regions of DNA C Intergenic Regions non coding DNA between genes NOT INTRONS These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute D Repetitive Sequences 1 Microsatellites micro short dinucleotide repeats copying errors 2 Genome wide Repeats relatively large as suggested by genome wide can be close together or dispersed can also be transposons which leads to the next point III Transposons jumping genes as opposed to jumping beans A Characteristics 1 Have personal gene encoding the transposase enzyme a Transposase enzyme cuts out and relocates transposons b May cause mutation duplication 2 Inverted terminal repeats recognized by transposase B Composite Transposons 1 Additional gene between insertion sequences 2 Often found in bacteria 3 Can allow antibiotic resistance C Cut and Paste Transposition 1 Exactly what it sounds like 2 Transposase removes transposon from one site and inserts it into a new target site 3 The DNA at the target site is split apart at the phosphodiester bonds leaving two sticky ends because DNA is double stranded and both strands split apart a Sticky ends are the genetic sequences at

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