Review Microscopy Always start with low power objective when focusing Final will include identification of specimens Fixed Protists slides are available for review Can you identify them from your notes Chlamydomonas Growth Curve Collect last data points Wash those tubes Scrub green ring Rinse tap water Remove marker with 100 Ethanol Place in bin Lab Chlamydomonas reinhardtii binomial species name Chlamydomonas Specimen available for review with microscope ni Single cell Flagella Green Plant Algae What did you measure A750 Convert to Cell Density with Standard Curve Lake Ecosystem All biotic factors All species All abiotic factors All in a geographically defined area Can independent variable factor be a limiting factor for algae growth Lab experiment Field experiment Lake Organisms Ciliates Paramecium Planarian Algae Black bellied ducks University lake Autotrophs and Heterotrophs Producers and Consumers Don t forget decomposers Fungi Algae Producers Chapter 12 Exercise 1 Bacteria Chapter 8 Exercise 2 Multiple trophic levels Examine microscopic samples Notice Domains Bacteria or Eukarya Notice Size Bacteria much smaller Notice Pigments Notice cell structures or identifying characteristics Can you use systematics to help you identify specimens Take notes so you can identify specimen in subsequent classes and exams Field Experiment Dependent Variable Chlorophyll What is Chlorophyll measuring Autotrophs Multiple Species of Algae Scientific paper Does change the growth of algae in lake bayou Systematics Producers Start with your kingdom tree completed in Systematics lab Unknown Common Ancestor No Introns Introns No Nucleus Single Cell or Colonial Nucleus Autotroph Bacteria Archaea Protista Plant Multi cellular Heterotroph Absorptive Fungi Ingestive Animal Build a Tree for Producers Use WHITE CARDS Traits on branches Organisms on tips Kingdom Colonial None Intron Nucleus Single colonial Multi cellular Producers Algae Now add Green and Purple Cards None Intron Nucleus Kingdom Bacteria Phycobilin Oscillatoria brevis Colonial Corallina officinalis Single colonial Protista Kerenia brevis Fucoxanthin Macrocystis pyrifera Multi cellular Plant Chara vulgaris Algae systematic tree following physical traits None Intron Nucleus Kingdom Bacteria Single colonial Protista Multi cellular Plant Oscillatoria brevis Colonial Corallina officinalis Phycobilin Chondrus crispus Kerenia brevis Thalassiosira Chara vulgaris Fucoxanthin Macrocystis pyrifera Systematic tree has free rotation around nodes Protista is a kingdom that is changing due to new molecular biology data None Intron Nucleus Kingdom Bacteria Oscillatoria brevis Colonial Thalassiosira Fucoxanthin Macrocystis pyrifera Single colonial Protista Multi cellular Plant Phycobilin Corallina officinalis Chondrus crispus Kerenia brevis Chara vulgaris Population Growth Curves How does the population grow in specific environments If you change the environment experimental variable does the population grow differently K Carrying Capacity Resource s limiting BIOL1209 graph this label should be vertical along axis Should include organism Homo sapiens on axis or figure caption Logistic Limited Resources Exponential Unlimited Resources Limiting Factor Growth Curve Population is at Carrying Capacity Notice we are comparing data at one time point y t i s n e D l l e C 35 2 5 30 25 1 5 20 15 10 0 5 5 0 0 Is your independent variable in the Chlamydomonas population growth experiment limiting Assay another concentration of the experimental variable 0 4 8 12 Time days The population can grow more if you give it more of the resource Limiting Factor Linear Trend Line The population can grow more if you give it more of the resource Linear trend line y mx b More growth by the factor of m y t i s n e D l l e C 2 5 1 5 2 1 0 0 5 0 5 10 15 Independent Variable Factor amt Limiting Factor Determine 2 ways y t i s n e D l l e C 35 2 5 30 25 1 5 20 15 10 0 5 5 0 0 y t i s n e D l l e C 2 5 1 5 2 1 0 0 5 0 4 8 12 Time days 0 5 10 15 Independent Variable Factor amt Both are ways of showing that more resource means more growth Lake data Is Lake data Linear Limiting Linear trend line is a Model R2 Statistical Analysis Way to reject your null hypothesis Ecosystem Why does a Blue Green Algae Bloom Cause Fish Kills Blue Green Algae an autotrophic bacteria produces oxygen by photosynthesis Lots and lots of tiny organisms Tiny With your table Write down several possible reasons for fish kills Be specific as possible Toxins Toxins can be the cause often not the primary cause Clue Remember this slide from population ecology e z i S n o i t a u p o P l s 1E 13 l a 9E 12 u d 8E 12 i v 7E 12 i d 6E 12 n I 5E 12 f o 4E 12 r 3E 12 e b 2E 12 m 1E 12 u N 0 0 10 20 30 40 50 60 70 80 Time days months years Generation Carrying Capacity K Limited Resources What is the limiting factor Nutrient Pollution Bluegreen algae overgrows Dense mats shade out other autotrophs Blue Green Algae exceeds carrying capacity dies Decomposition uses more oxygen than was produced by photosynthesis Organisms needing oxygen die LSU Lake System and Fish Kills LSU lake system had a fish kill within the first 10 years of dredging 1 Reported Dissolved oxygen of 2 6 ppm for 1948 fish kill 1 Dissolved oxygen is one of the primary indicators of Lake Health 1 Mary Grace Curry 1973 LSU Dissertation Reliable Resources Peer Reviewed Scientific Journals Gold Standard Evaluated by experts Web Sites need evaluation gov org com Mission Statement stated purpose Look for evidence of bias Credentials of authors References In class assignment Groups submit one document Complete your Chlamydomonas Population Growth Curve graphs Highlight one for comparison to Lake data Open WA Template Ecology Graphs Graph from last week s writing assignment for presentation Highlight one for comparison to Lake data Reference search for abiotic factors and aquatic ecology Websites Why reliable Scientific journal article reference Check each others work Use pictures and words to create slides answering the questions asked Apply acquired knowledge to the Food web Use arrows to indicate if the groups in the food web will increase or decrease Apply acquired knowledge How will the lake restoration project affect your independent variable