BIOL 1208 Fall 2025 Techniques In Class Assignment Name Lab Partner Name s Remember Although you work with partners for lab experiments written assignments must be completed independently and answered in your own words All text must be able to be processed in Turnitin so do not use screenshots etc See the syllabus for more information 1 Insert a photo of your completed Serological Pipettes Exercise here EXERCISE I PIPETTES AND PIPETTING 2 Insert a photo of your completed Micropipette Exercise card here Crop photo and resize to 2x3 3 What is the range limit of the following pipettes P100 micropipette 100 microliters P1000 micropipette 1000 microliters 4 What are the purposes of the first and second stops of a micropipette First used to draw up the precise amount of liquid needed Second used when dispensing liquid to make sure all the previously drawn liquid has been dispensed For Questions 5 6 Using the appropriate micropipette dispense 250 L of water onto the scale Follow the scale directions at your bench to Tare Zero scale before dispensing water into weigh boat 1 7 A lab technician dispenses liquid onto a scale and the readout volume is 0 05 grams How many microliters was 5 List micropipette P100 or P1000 selected P1000 6 Scale readout of volume in grams 0 250 BIOL 1208 Fall 2025 their pipette set to 5 microliters 8 If an object is orange which colors are being absorbed ROYGBV Transmitted EXERCISE II SPECTROMETRY Absorbed all other colors Transmitted Orange 9 What is directly measured by a spectrometer The amount of light a substance absorbs 10 What is the purpose of a blank in spectrometry To recalibrate the spectrometer 11 What are the units for absorbance For concentration Absorbance ODU Concentration M 12 Based only on your observation of the colors of the solutions which cobalt chloride CoCl2 sample was more A COBALT CHLORIDE concentrated A or B How do you know Sample A because it has a more vibrant red color 13 Provide the absorbance values from the spectrometer reading for both solutions Use the graph to visually estimate the concentration Use the standard curve equation to calculate the concentration Remember solve for X Show your work for at least one calculation CoCl2 Solution A o Absorbance ODU 1 11 odu o Estimated Concentration M 0 2 M o Calculated Concentration M 0 2186M CoCl2 Solution B o Absorbance ODU 0 292 odu o Estimated concentration M 0 056 M o Calculated Concentration M 0 0574 M 2 Show your calculation here BIOL 1208 Fall 2025 B CRYSTAL VIOLET FADING OVER TIME 14 How do you expect the color intensity of crystal violet to change over time when combined with sodium hydroxide NaOH Based on Beer s law what do you predict will happen with the absorbance values of this solution over time I believe the vibrancy of the CV solution will diminish Based on Beer s law there should be a reduction in absorption values 15 Complete Table 1 4 be sure to include a complete caption include the standard curve equation Show your work for at least one calculation Table 1 4 Type caption here Time s Absorbance ODU 0 30 60 90 0 123 0 124 0 118 0 116 Concentration mM Estimated Calculated 0 024 0 024 0 023 0 023 0 0241 0 0243 0 0231 0 0227 Show your calculation here 16 Does your data in Table 1 4 support your predictions color intensity and absorbance from 14 Explain Yes just not as precisely as my calculations Save your completed assignment as a pdf with the following file name Your first initial and last ASSIGNMENT SUBMISSION name Assignment name 3 o To convert to a PDF in Word see 4 here o If file too large use a PDF compressor PDF2GO Adobe BIOL 1208 Fall 2025 Submit your completed assignment via the assignment s submission link on Moodle o You can submit assignments as many times as you want to before the due date o After the due date you can only submit assignments if you have requested and received an extension by your Open the submission and check that it was uploaded to the correct link is the correct file is accessible and is not TA blank See the Due Date Schedule on Moodle 4