Self Assessment Lecture 34: Nucleotide Catabolism1. Please state the function of the following: a. Nucleotidase___ Removes the phosphate from a nucleotide to form a nucleoside__b. Nucleoside Phosphorylase___Forms a free base and ribose 1-P from a nucleoside _c. Phosphoribomutase_______________Forms ribose 5-P from ribose 1-P___2. Catabolism of both purine bases forms this common intermediate, which is also a nitrogenous base:_________Xanthine______________________________3. What is the final product purine catabolism?______ Urate________________4. What advantage does the high serum urate seen in humans offer? _______________ Urate is a potent antioxidant. _________________________________________________5. What is the disadvantage of having serum urate concentrations that are too high?__________Urate can precipitate to result in gout ___ 6. How does allopurinol function?__________ An inhibitor of xanthine oxidase that decreases urate production ___________________________7. What is the most prevalent cause of SCID (severe combine immunodeficiency)? ______ Deficiency in adenosine deaminase ____________________________________________________8. What is the handedness (R or L) of the following DNAs A=R___B=R ___Z=L __9. Most DNA under physiologic conditions is in what form, A, B, or Z?__ B _____.10. Number of bases in one 360˚ turn of DNA=__10.4 ___Angstroms in one turn=___34 ___11. Differenced in B and _{A/Z}___ arise due to DNA sequence; runs of dCGCGCGCG can favor this DNA conformation.12. Circular genomic DNA is found in _{prokaryotes, eukaryotes, both}_; circular extra-genomicDNA is found in _{prokaryotes, eukaryotes, both}_.13. Please place the following in order, from most simple to most complex higher order eukaryotic structure:__________ BFAEDC ______________a. DNA 10 nm fiber b. DNA helixc. highly condensed metaphase chromosomed. loops attached to scaffold proteinse. solenoid f. nucleosome14. This genomic DNA component in eukaryotes consists of 147 base-pairs of DNA wrapped ~1.7 times around a histone core.______ Nucleosome _________________15. Histone cores are comprised of: 2(H1, H2A, H2B H3, H4, H5, H6 H7) (choose all that apply)Self Assessment Lecture 34: Nucleotide Catabolism16. Higher order structure of DNA is promoted through electrostatic interactions between _________Lysines in histone tails and phosphate groups in the DNA backbone__17. Histone Transacetylase is an enzyme that transfers an acetyl group to ________Lysines in histone tails_________________ and _{promotes, inhibits}_ access to the DNA 18. What class of enzymes reverses the modification seen in Q18?______________ Histone Deacetylase (HDAC)19. Which of the following is NOT a valid histone modification:a. ADP-ribosylationb. phosphorylationc. methylationd. ubiquitination /sumolyation e. acetylationf. None of these are valid histone modifications.g. All of these are valid histone
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