Self%assessment%Lecture%35%Key%Replication%Mutation%and%Repair%%Self%Assessment%Lecture%35%1. Silent%mutations:%a. Can%occur%in%the%coding%region%b. Can%occur%in%the%intron%c. Can%occur%in%the%5´and%in%the%%3´nonBcoding%regions%d. All%the%above%(note:'why'can'a'mutation'in'the'coding'region'not'result'in'a'phenotypic'change?)%2. A%change%in%only%one%amino%acid%is%called%a%___point%mutation____%3. A%mutation%which%results%in%a%change%from%A%to%T%{transition/transversion}%4. A%mutation%which%results%in%a%change%from%A%to%G{transition/transversion}%5. In%a%Missense%mutation:%__The%mutation%results%in%a%change%of%amino%acid%encoded%%6. In%a%NonBsense%mutation___%The%mutation%results%in%a%stop%codon%and%so%a%truncation.%7. If%a%mutation%results%the%insertion%or%deletion%of%only%one%nucleotide%in%the%coding%region,%it%will%generally%have%a%much%lesser%effect%on%the%phenotype%than%if%the%mutation%is%a%deletion%or%insertion%of%15%deoxynucleotides.TF%8. Please%name%two%chemicals%that%were%discussed%which%intercalate%into%the%DNA%a. ____Acridine%Orange_______________________________________________%b. ____Ethidium%bromide_______________________________________________%9. Intercalators%in%Q10%generally%cause%what%type%of%mutation?____frame%shift______%10. If%a.%in%the%figure,%right,%is%the%normal%chromosome,%please%identify%b.%and%c.%a. ___%inversion______________%b. __%translocation%_______________________%11. Both%b%and%c%arise%from%%a. DNA%Single%strand%break%b. DNA&Double&strand&break&12. Huntington’s%disease%is%a%defect%in%what%protein?%___Huntingtin____%13. Please%describe%the%type%of%mutation%that%results%in%%Huntington’s%disease%insertion%of%a%tridNTP%repeat%t ha t %results%in%expansion%of%a%polyglutamine%tract%in%the%huntingtin%protein_____________________%14. Please%describe%anticipation%with%as%it%pertains%to%Huntington’s%disease%%in%anticipation%the%severity%increases%and%age%of%symptom%onset%decreases.%%15. DNA%bases%are%predominantly%in%the%{enol/keto}%configuration,%but%this%process%can%cause%a%shift%in%double%bond%location%to%produce%the%{enol/keto}%form:________________tautomerization_______________________%16. Alfatoxin%s%a%compound%found%in%peanut%mold,%and%is%itself%a%potent%carcinogen.%TF%17. During%mismatch%repair,%how%is%the%newly%synthesized%strand%identified%from%the%parent,%template%strand?%_________%Only%the%old%strand%is%methylated____%%%%%%%a.%%%%%%%%%%%%b.%%%%%%%%%%%c.%%Self%assessment%Lecture%35%Key%Replication%Mutation%and%Repair%%18. Please%place%the%following%in%correct%order%for%mismatch%repair%in%E.coli._%%c,%a.,%d,%e,%b%__%a. MulL%b. DNA%Pol%III%c. MutS%d. MutH%e. Exonuclease%I%%19. Humans%can%repair%thymine%dimers%through%use%of%%a. the%enzymeDNA%photolyase%b. nucleotide%excision%repair%c. both%a.%and%b.%are%used%%20. What%is%an%AP%site,%and%what%enzyme%creates%it?%_____apurinic,%apyrimidinic%site%_created%through%action%of%glycosylase%%which%cleaves%off%the%base.%%21. DNA%Recombination%does%which%of%the%following?:%a. Repairs%DNA%%b. Increases%diversity%in%offspring%c. Generates%antibody%diversity%d. Introduces%new%genes%e. All%but%c%f. a.%–%d.%are%all%true%22. Please%put%the%event%of%homologous%recombination%in%order:___d.,%f.,%a,%c.,%b.,%e.%%a. 3´%Strand%invasion%b. Branch%migration%c. Ligation%of%the%3´end%of%one%homologous%strand%to%it’s%homolog%in%the%other%double%strand%d. Chromosome%synapsis%e. Duplex%resolution%to%form%two%new%double%strands%of%DNA%f. SS%nicking%%(this%is%cleaving%one%strand%of%a%duplex%DNA)%23.
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