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MSU BMB 401 - Lecture_36_Self_Assesment_KEY_Transcript

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Lecture'36'Self'assessment:'Transcription''1. Please(identify(the(following( (a. Coding(Strand(b. Template(Strand(2. In(this(stage(of(transcription,(the(DNA(is(unwound,(and(the(RNA(polymerase,(with(associated(transcription(factors(is(present(at(the(promoter,(but(nucleotides(have(not(been(polymerized(as(yet.(__Initiation________(3. Please(explain(the(polymerase(reaction(and(state(the(process(that(drives(it(forward:((_____The*incoming*alpha*phosphate*undergoes*a*nucleophilic*attack*by*the*3’*OH*of*the*preceding*nucleotide;*the*phosphoanydride*bond*is*made*and*pyrophosphate*is*given*off*as*a*leaving*group.(_________(4. What(effect(does(Topoisomerase(II(have(on(transcription:({assists/inhibits}(5. ….(and(how(does(it(have(this(effect?({inducing/removing}*negative(supercoils.*6. In(prokaryotes,(the(core(RNA(polymerase(is(made(up(of(what(subunits?_α2*β*β’__(7. σ*Factor(serves(what(purpose?(Provides*specificity*to*the*polymerase*:*σ*decreases*core*affinity*for*random*DNA*but*at*the*same*time*allows*the*core*to*recognize*specific*promoter*sequences(((8. Please(state(the(parts(of(a(prokaryotic(promoter(necessary(for(attracting(the(RNA(polymerase_P10,*and*P35*regions*and*the*intervening*space.*You*should*know*what*a*consensus*sequence*is,*what*this*means*for*promoter*strength,*what*recognizes*the*promoter.((9. What(is(the(reason(for(this(spacing(of(these(elements?__To*optimally*place*the*P10*and*P35*regions*into*the*binding*spaces*of*the*sigma*factor*for*optimal*DNAPprotein*contatcts_____________________________________((10. What(is(it(in(eukaryotes(that(attracts(the(RNA(polymerase(to(promoters(in(eukaryotes?_______TFIID_________________________________________________________________((11. The(UP(element(is(found(where?(___________Upstream*of*the*promoter*of*highly*transcribed*genes.*You*should*know*what*these*genes*were*discussed*in*lecture.*__((12. Which(of(the(following(are(FALSE?(Choose(all(that(apply.(a. The'UP'element'is'part'of'the'core'promoter'in'highly'expressed'genes''b. TFIID(Binds(to(the(RNA(polymerase(itself(c. The(taata(box(is(rich(in(A(and(T(dNTs,(which(provide(the(flexibility(necessary(for(this(DNA(region(to(wrap(around(and(stabilize(the(polymerase(at(the(promoter(to(increase(the(chances(of(initiation(occurring.(d. The'UP'element'is'a'transcription'factor'in'prokaryotes.'ATCGGGCTAGCATCGGGCTAGCATCGGGCTAGC TAGCCCGATCGTAGCCCGATCGTAGCCCGATCG 5’ 3´ 3’a____ 5´b____Lecture'36'Self'assessment:'Transcription''13. Name(two(means(by(which(termination(in(prokaryotes(occurs(a. ___GC*Stem*–loop._____Followed*by*polyA*and*polyU*region_________((b. ___Rho*factor*mediated*You*should*know*how*this*works!*___(_____________((14. Which(of(the(following(are(true?(a. Most(prokaryotic(mRNAs(are(usually(postYtranscriptionally(modified((b. Most'prokaryotic'RNAs'are'polycistronic'–'one'promoter'serves'to'the'transcription'of'an'RNA,'which'is'comprised'of'several'protein'coding'sequences.''c. tRNAs'in'prokaryotes'are'transcribed'in'tandem'and'clipped'from'the'larger'RNA'transcript.'d. rRNAs'and'tRNAs'in'prokaryotes'are'transcriptionally'modified.'(15. Concurrent(transcription(and(translat ion(of(a(gene(can(occur(in({prokaryotes/eukaryotes}((16. Please(state(the(function(of(each(Eukaryotic(RNA(polymerase(a. RNA(Pol(I(transcribes:__________rRNAs(5.8S,(18S,(28S_____________________________(b. RNA(Pol(II(transcribes:____mRNA(precursors(ans(snRNAs________(c. RNA(Pol(III(transcribes:___________tRNAs(and(rRNA(5S______((17. (Which(of(the(following(are(true(of(TBP?(TBP:(a. is(found(only(in(Pol(II(transcriptional(initiation(complexes(b. is(symmetric:((c. provides'directionality'for'further'transcription'factors'd. provides'directionality'for'transcription.'e. bends(DNA(at(the(CAAT(Box(18. Please(name(the(members(of(the(basal(transcription(Pol(II(complex:(TFIIA,'TFIIB,'TFIID,'TFIIE,'TFIIF,'TFIIH'(19. (Which(of(the(following(is(FALSE?(a. TBP(is(part(of(TFIID(b. TFIIA(binds(to(TBP(and(stabilizes(TBP(binding(at(the(promoter((c. TFIIA'recruits' RNA'polymerase'II,'with'the'help'of'TFIIF'd. TFIIH(phosphorylates(the(Pol(II(CYTerminal(Domain(e. TFIIH(contains(a(helicase,(and(kinase(necessary(for(promoter(escape(f. TFIIE(and(TFIIH(are(both(necessary(for(promoter(escape((20. Please(name(the(modifications(present(in(eukaryotic(mRNAs(at(the(3’(and(5’(ends_____5’MethylGuanosine*Cap*and*3’PolyPA*tail**P*you*should*know*the*functions*of*these*structures!((21. What(is(the(signal(in(eukaryotes(to(stop(mRNA(transcription?_mRNA*Cleavage*Signal'(Lecture'36'Self'assessment:'Transcription''(22. ({RNA'Pol'I/RNA(Pol(lI/RNA(Pol(III}(in(the(nucleolus(synthesizes(a(long(transcript(that(will(be(modified(and(clipped(to(form(____rRNAs_____________________________________((23. (The(clipping(process(referred(to(in(Q22(and(splicing(are(the(same(process.((T'F((24.


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MSU BMB 401 - Lecture_36_Self_Assesment_KEY_Transcript

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