BCHM 3050 1st Edition Lecture 35Outline of Last Lecture I. Position of Four Complexes in Cristae MembraneII. Important Biomolecules Involved in Respiratory Electron TransportIII. Complex 1IV. Complex 2V. Complex 3VI. Complex 4VII. Reduction Potentials Along Electron Transport ChainVIII. “Poisons” are Inhibitors of Respiratory Electron TransportOutline of Current Lecture I. “Alternate Oxidase” in PlantsII. Aerobic Respiration: ATP SynthesisIII. ATP Synthesis TerminologyIV. Three Sites in Electron Transport with Sufficient Energy Yield for ATP SynthesisV. Stoichiometry of Respiratory Electron TransportCurrent LectureI. “Alternate Oxidase” in Plantsa. Skunk Cabbage smells due to heavy smell from sulfurb. They have complex 1 and 2 but not complex 3 or 4c. CoQ gives enzymes directly to oxygen so we cannot inhibit this pathways with cyanidei. They have an alternate oxidased. An alternate oxidase à lesser ATP produce and the extra energy is lost as heat àincrease the temperature so these plants always feel warmThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.e. Reoxidation of electron carriersf. Alternate oxidase have more volatile odors to attract insect pollinators, less ATP, and more heat releasedII. Aerobic Respiration: ATP Synthesisa. For every NADH that gives up its electrons à harvest about 2.5 molecules of ATPb. For every FADH2 that gives up its electrons à harvest about 1.5 ATP moleculesc. In theory, you should get 28 ATP molecules from the electron transport chain ((10 x 2.5) + (2 x 1.5)), plus 2 ATP from glycolysis, plus 2 ATP from TCA à 32 molecules of ATP from every glucose molecule synthesizedi. This is not always true, usually a number between 25 and 30III. ATP Synthesis Terminologya. Organic phosphate + ADP = ATPb. Substrate level phosphorylation – happens on an enzymei. Phosphoglycerate kinase and pyruvate kinase use substrate level phosphorylation in glycolysisii. Succinal CoA synthetase does this in TCAc. Oxidative phosphorylation – oxidative processd. Photophosphorylation - Coupling of ATP synthesis to drive photosynthesisIV. Three Sites in Electron Transport with Sufficient Energy Yield for ATP Synthesisa. 1 NADH in the mitochondria becomes NAD+ à accumulate 10 protons in the intermembrane space after gone through all 4 complexesb. 1 FADH2 becomes FAD (in complex 2, the succinate dehydrogenase complex)à accumulate 6 protons (4 from complex 3 and 2 from complex 4)c. FAD does not go through complex 1V. Stoichiometry of Respiratory Electron Transporta. After electron transfer, increasing number of protons in the intermembrane spaceb. As all the NADH and FADH2 continue, protons continue to accumulatec. About 104 protons in the intermembrane space for 1 molecule of glucosed. Totals: 8 NADH à 80 protons; 2 FADH2 à 12 protons; 2 NADH from cytoplasm after glycolysis (go through complex 2, 3, and 4, not complex 1) à (2 x 6 protons)= 12 protonse. 1 glucose = 104 protonsf. NADH goes through complex 1, 2, 3, 4 à 10 protons per 1 NADHg. FADH2 goes through complex 2, 3, 4 à 6 protons per 1
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