BS 161 1st Edition Lecture 39Outline of Last Lecture I. BiotechnologyOutline of Current Lecture II. Biotechnology continuedCurrent Lecture-RFLP: restriction fragment length polymorphism-main steps in finding a disease-related gene in the human genome without previous knowledgeof the gene:-find a large family with multiple affected members, use DNA from this pedigree-find a restriction fragment length polymorphism (RFLP) that always is present in the affected but not the normal individuals-transgenic organisms: fourth use of cloned DNA-genetically engineer the cloned DNA for transgenic cells (organisms) or gene therapy-transgene: a recombinant DNA inserted into the genome -used for…-research to study the gene-in agricultural biotech to improve yield-in biomedical applications to make a protein-in gene therapy to correct mutations-examples of transgenic gene therapy:-adding vitamin A to rice-use gene therapy to treat x-linked immune deficiency-ex: severe combined immunodeficiency (SCID)-main steps of the strategy in human gene therapy: -clone normal functional gamma-C gene into a retroviral vector-transform patient’s stem cells for lymphocytes with the transgenic retrovirus to make them transgenic-stem cells: renewable dividing cells which give rise to a differentiated cell and another stem cell; precursors of T cells and other blood
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