Chapter 9 MULTIPLE CHOICE Choose the one alternative that best completes the statement or answers the question 1 A restriction fragment is A cDNA B a segment of mRNA C a gene D a segment of DNA E a segment of tRNA Answer D 2 PCR can be used to identify an unknown bacterium because A the RNA primer is specific B DNA polymerase will replicate DNA C DNA can be electrophoresed D all cells have DNA E all cells have RNA Answer A 3 The Human Genome Project which was completed in 2003 was focused on A determining all of the proteins encoded by the human genome B finding a cure for all human genetic disorders C cloning all of the genes of the human genome D determining the nucleotide sequence of the entire human genome E identifying all of the genes in the human genome Answer D 4 The use of suicide genes in genetically modified organisms is designed to A prevent the growth of the modified organisms in the environment B provide for resistance of the modified organisms to pesticides C kill the modified organisms before they are released in the environment D delete genes necessary for modified organism s growth E provide a means to eliminate non modified organisms Answer A 5 If you have inserted a gene in the Ti plasmid the next step in genetic engineering is A transformation of an animal cell B inserting the Ti plasmid into Agrobacterium C transformation of E coli with Ti plasmid D splicing T DNA into a plasmid E inserting the Ti plasmid into a plant cell Answer B 1 6 The following are steps used to make DNA fingerprints What is the third step A Add stain B Perform electrophoresis C Digest with a restriction enzyme D Collect DNA E Lyse cells Answer C Figure 9 2 7 In Figure 9 2 the enzyme in step 2 is A reverse transcriptase B spliceosome C DNA polymerase D DNA ligase E RNA polymerase Answer A 2 8 Assume you have discovered a cell that produces a lipase that works in cold water for a laundry additive You can increase the efficiency of this enzyme by changing one amino acid This is done by A selection B selective breeding C enrichment D irradiating the cells E site directed mutagenesis Answer E 9 Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases such as hepatitis B A DNA fingerprinting B reverse transcriptase PCR rtPCR C tumor inducing plasmids Ti plasmids D complementary DNA cDNA E RNA interference RNAi Answer E 10 The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells A new rapid diagnostic test to detect human papilloma virus HPV DNA before cancer develops is done without microscopic exam The steps involved in this FastHPV test are listed below What is the second step A Add enzyme linked antibodies against DNA RNA B Add enzyme substrate C Lyse human cells D Add an RNA probe for HPV DNA E The order is unimportant Answer D 11 The restriction enzyme EcoRI recognizes the sequence G AATTC Which of the following is TRUE of DNA after it is treated with EcoRI A All of the DNA fragments will have single stranded regions ending in G B All of the DNA fragments will have single stranded regions ending in AA C All of the DNA will have blunt ends D All of the DNA will be circular E Some of the DNA will have single stranded regions ending in AA and others will end in G Answer B 12 A source of heat stable DNA polymerase is A Saccharomyces cerevisiae B Thermus aquaticus C Agrobacterium tumefaciens D Bacillus thuringiensis E Pseudomonas Answer B 3 Figure 9 5 13 Which of the following methods would be used to introduce the plasmid shown in Figure 9 5 into E coli A Ti plasmids and Agrobacterium B gene guns C microinjection D transformation Answer D 14 Which of the following techniques is NOT used to introduce recombinant DNA into plants A electroporation B protoplast fusion C Ti plasmids and Agrobacterium D gene guns E microinjection Answer E Figure 9 3 15 The figure at the left in Figure 9 3 shows a gene identified by Southern blotting What will a Southern blot of the same gene look like after PCR A a B b C c D d E e Answer D 4 16 Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures A self replication B has a selectable marker C large size D circular form of DNA or integrates into the host chromosome E may replicate in several species Answer C Figure 9 1 17 In Figure 9 1 after digestion with the appropriate restriction enzyme what is the smallest piece containing the ampicillin resistance amp gene A 0 17 kbp B 0 25 kbp C 1 08 kbp D 1 50 kbp E 3 00 kbp Answer D 18 The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes A the recombinant cell dangerous B the recombinant cell unable to survive C direct selection possible D replica plating possible E All of the answers are correct Answer C 19 A population of cells carrying a desired plasmid is called a A vector B library C clone D PCR E Southern blot Answer C 5 20 Which of the following is NOT an agricultural product made by DNA techniques A pectinase B nitrogenase nitrogen fixation C glyphosate resistant crops D Bacillus thuringiensis insecticide E frost retardant Answer A 21 The reaction catalyzed by reverse transcriptase is A DNA DNA B tRNA mRNA C mRNA cDNA D DNA mRNA E mRNA protein Answer C 22 Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease A DNA fingerprints B restriction fragment length polymorphisms C reverse transcriptase PCR rtPCR D DNA fingerprings and restriction fragment length polymorphisms E DNA fingerprings restriction fragment length polymorphisms and reverse transcriptase PCR rtPCR Answer E 23 The random shotgun method is used in A genome sequencing B transforming plant cells with recombinant DNA C forensic microbiology D amplification of unknown DNA E RFLP analysis Answer A 6 Figure 9 5 24 In Figure 9 5 the marker genes used for selecting recombinant DNA are A HindIII BamHI and EcoRI B ampR and lacZ C ampR and ori D ori E lacZ and ori Answer B Figure 9 1 25 How many pieces will EcoRI produce from the plasmid shown in Figure 9 1 A 1 B 2 C 3 D 4 Answer B 7 E 5 26 The study of genetic material taken directly from the environment is A bioinformatics B forensic microbiology C reverse genetics D proteomics E metagenomics Answer E 27 Self replicating DNA used to transmit a gene from one organism to another is a A library B vector C Southern blot D clone E PCR Answer B 28 The following