TAMU BIOL 111 - 20_Lecture_Presentation (64 pages)

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20_Lecture_Presentation



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20_Lecture_Presentation

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Pages:
64
School:
Texas A&M University
Course:
Biol 111 - Introductory Biology I

Unformatted text preview:

CAMPBELL BIOLOGY TENTH EDITION Reece Urry Cain Wasserman Minorsky Jackson 20 DNA Tools and Biotechnology Lecture Presentation by Nicole Tunbridge and Kathleen Fitzpatrick 2014 Pearson Education Inc Biotechnology is the manipulation of organisms or their components to make useful products The applications of DNA technology affect everything from agriculture to criminal law to medical research 2014 Pearson Education Inc Concept 20 1 DNA sequencing and DNA cloning are valuable tools for genetic engineering and biological inquiry The complementarity of the two DNA strands is the basis for nucleic acid hybridization the base pairing of one strand of nucleic acid to the complementary sequence on another strand Genetic engineering is the direct manipulation of genes for practical purposes 2014 Pearson Education Inc DNA Sequencing Researchers can exploit the principle of complementary base pairing to determine a gene s complete nucleotide sequence called DNA sequencing The first automated procedure was based on a technique called dideoxy or chain termination sequencing developed by Sanger 2014 Pearson Education Inc Figure 20 2 a Standard sequencing machine b Next generation sequencing machines 2014 Pearson Education Inc Figure 20 3 Technique DNA template strand 5 C T G A C T T C G A C A 3 A 5 Primer 3 T G T T 5 DNA polymerase dATP ddATP dCTP ddCTP dTTP ddTTP dGTP ddGTP P P P Shortest dd T G A A G C T G T T Longest labeled strand Detector Laser Shortest labeled strand Results Last nucleotide of longest labeled strand Last nucleotide of shortest labeled strand 2014 Pearson Education Inc P P P G Labeled strands DNA template C strand T G A dd G C dd A A T dd A T A A C G G G 3 dd G G dd C C C C C A T T T T T C G G G G G A T T T T T 3 A T T T T 5 T Direction of movement of strands Dideoxyribonucleotides fluorescently tagged Deoxyribonucleotides G A C T G A A G C dd C T G A A G C T G T T dd A C T G A A G C T G T T G dd G 3 A C T G A A G C T G T 5 T Longest Next generation



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