Lab Review Lab 1 The Discovery Process 1 What type of microscope is used to view minute organisms such as these flagellates from a termite s gut compound microscope 2 for What type of scope is shown Is it used larger or smaller items dissecting or steromicroscope Used for larger specimens 3 Identify the iris diaphragm What type of microscope is shown compound scope 4 Identify objectives stage nosepiece slide holder What type of microscope is shown compound microscope 5 As magnification increases the amount of light needed to view the specimen and the field of view becomes increases smaller 6 In microscopy as magnification increases depth of field How do you adjust the focal plane at high magnification decreases with the fine focus ONLY Lab 2 Properties of Water 1 In this demonstration what s the solvent and what s the solute water is the solvent the solutes are the pigment particles in the ink 2 What type of carbohydrates does testing with Lugol s solution detect starch specifically amylose 3 The process by which cells lose water in a hypertonic solution is called plasmolysis 4 Which has a higher concentration of H ions an acidic solution or a basic one acidic 5 What property of water allows these insects to walk on its surface high surface tension 6 In a hypertonic solution water is moving the cells out of 7 Are these plant or animal cells Identify some of the structures plant nucleus chloroplasts cell wall vacuole 8 Why is Elodea Anacharis a commonly placed in fresh water aquaria it releases oxygen as a product of photosynthesis which is essential to keep fish healthy 9 Name two ways the green dots support life on earth site of photosynthesis which produces O2 and sugar O2 is essential for aerobic respiration Sugar is basis of most food webs 10 What is the evolutionary history of chloroplasts in plant cells believed to be originally cyanobacterial endosymbionts Lab 3 Biomolecules 1 What does a positive test with each of these three reagents indicate Benedict s reducing sugar Lugol s starch Biuret peptide bonds proteins 2 What color is a negative test with Benedict s solution What color is positive Negative is pale blue positive depends on amount of sugar present high concentrations and with heat a positive 3 What type of carbohydrates does testing with Lugol s solution detect starch specifically amylose 4 In the presence of peptide bonds Biuret reagent changes from to light blue to purple 5 Which of these tests shows the presence of an amino acid positive ninhydrin 6 What does this indicator test for and what color denotes a positive result amino acid purple or yellow 7 What type of molecule is this The H C O group is terminal so this is an aldehyde 8 Based upon this Lugol s test is starch stored in onion cells no 9 What sort of compound is shown ketone the C O is in the middle of the molecule 10 What functional group is present in this molecule carboxyl OH C O What sort of chemical compound is this 11 amine the nitrogen is a giveaway 12 In lab the positive Sudan IV test indicates the presence of what type of biomolecule lipid 13 Identify the functional groups A ketone B amine C aldehyde D carboxyl E hydroxl Lab 4 Enzymes Identify sample compartment wavelength selector mode selection screen where results appear 2 Refer to the lab manual and identify the labeled structures Lab 5 Cells 1 What does each of these reagents do in a Gram staining procedure What other reagent is needed Crystal violet stains peptidoglycan iodine fixes violet alcohol decolorizes by removing outer layer 2 In what order are these used violet water iodine water alcohol water safranin water 3 Identify this organism How does it move Paramecium cilia 4 Describe how the form of these cells fit their function mobile sperm cells must swim to the egg flagella propels the nuclear material forward 5 6 Is this an animal or a plant cell How can you tell Answer animal human no cell wall Identify the large dark mass in the center of the cell nucleus 7 What is the central empty feature in the leftmost cell Is this a plant or animal cell vacuole plant 8 Identify plasma membrane nucleus Golgi apparatus ribosome mitochondrion centrioles flagellum 9 To what domain do these organisms belong Bacteria 10 Which antibiotic appears to be most effective against the bacterial culture in this test plate How can you tell C it has the largest zone of inhibition 11 What is the function of bacteria flagella locomotion 12 What is the name for the spiralshaped bacterium spirillum 13 Describe the characteristic shape of these organisms bacillus or bacilli 14 What is the morphological description for this bacteria Is it Gram or Gram bacillus Gram 15 Correctly describe the morphological shape of this bacteria coccus or cocci 16 Why did these cells stain purple during Gram staining cell wall has complex layer of peptidoglycan 17 How do you know that this organism is a eukaryote it has a large nucleus 18 To what Domain does this organism belong Bacteria 19 What is the difference between these cells and human red blood cells human red blood cells lack nuclei 20 What are the thread like extensions of this cell and what are they used for Flagella locomotion 21 Identify the domain and next lowest taxon of these organisms Domain Bacteria Cyanobacteria 22 Identify the darkly stained organelle in these cells What color is the cytoplasm nucleus and pink 23 Why isn t this a Eukaryotic organism The cells lack nuclei or membrane organelles 24 Name three environmental functions of these organisms produce oxygen absorb excess carbon dioxide primary producers for marine ecosystems Lab 6 Cellular Metabolism 1 Flask water sugar yeast Cylinder bromothymol blue Explain color yeast respiring CO2 given off turns cylinder acid indicator goes to yellow 2 of 3 When do you release the plunger for the last time AFTER the pipette tip has been lifted out of the container you dispensed into otherwise you may aspirate some the container contents What are these and what do they do Pi pumps or pipette pumps used to draw up liquid in glass pipettes and dispense it 4 5 Identify plunger tip discard button adjustable volume control What does the red staining positive tetrazolium TTC test indicate TTC tests for respiration positive test means seeds are alive 6 Which of the organisms in this photo undergo cellular respiration to produce carbon dioxide All of them Plants respire just as animals do 7 What type of gland opens into a hair follicle sebaceous gland 8 What peculiar
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