MCB 450 1st Edition Lecture 26Outline of Last Lecture I. Ribosomal subunitsII. Initiation complex formationIII. ElongationIV. TerminationV. Prokaryotic vs. eukaryotic protein synthesisVI. Synthesis of secreted proteinsVII. Regulation of protein synthesisVIII. MutationsOutline of Current Lecture I. Restriction Enzymes and their usesII. Cloninga. definition of plasmidb. construction of a chimeric plasmidc. transformationCurrent LecturePeptide bond formation by peptidyl transferase-Green amino acid which is at the A site will enter the P site-Will attack the ester bond that attaches the blue amino acid to its tRNA and then that dipeptide remains attached to the green tRNA and is present at the P siteEF-G Mimics EF-Tu-tRNA-EF-Tu is the factor that delivers the tRNA to the A siteAn Active Ribosome-Purple amino acid will move into the P site and attack the ester bond between the tRNA and the blue and green amino acids forming a tripeptideTranslation Termination-First picture the A site is free and P site is occupied-RF -- releasing factor will go to A site and will release the peptide from the P siteTranslation in Eukaryotes-Monocistronic (one coding region)Synthesis of secretory and membrane proteins-Translation occurs in different regions of the cells-SRP -- signal recognition particle These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.Regulation of Protein Synthesis-One way to regulate translation is a specific region of the untranslated mRNA (in)eukaryotes)oHairpin structure (iron-response element_-Can't have ferritin loose (must be bound) because it can become an oxidant-PalindromeMutation and Mutagenesis Some Terminology-Terms that are often confused with each otherInsertion/Deletion Mutations-Insertions or deletions of a number of bases that are not divisible by 3 cause a "frameshift"-Mutations are how cancer developsRestriction Endonucleases: CcoRI-AATT is a palidrome-The palindrome is cut out-And makes up the sticky endsOther Common Restriction Enzymes-Don't have to remember names or sequences just have to understand basics about themlike the fact that they are a palindromeSouthern Blotting-DNA probe revealed for Gene A-Southern blotting is how you do DNA
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