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UIUC MCB 450 - Protein Synthesis

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MCB 450 1st Edition Lecture 25Outline of Last Lecture I. Ribosomal RNA processingII. tRNA processingIII. mRNA processingIV. RNA binding proteinsV. The genetic codeVI. Wobble hypothesisVII. tRNA structureVIII. tRNA anticodon binding to codonIX. Aminoacyl-tRNA synthetaseOutline of Current Lecture I. Ribosomal subunitsII. Initiation complex formationIII. ElongationIV. TerminationV. Prokaryotic vs. eukaryotic protein synthesisVI. Synthesis of secreted proteinsVII. Regulation of protein synthesisVIII. MutationsCurrent LectureWobble Hypothesis• Only the third pair is "wobbly" and not as strict with which base it pairsWobble hypothesis for degeneracy of the genetic code• G is the only base that doesn't pair with ICovalent linkage of amino acid to cognate tRNA• Ester bond between amino acid and tRNAFormylation of met-tRNA, in E. coli• Methionine only has one codon but has two tRNACharging of tRNAs• Need to have a way to correct this error this is done by synthetaseRecognition sites on tRNA for synthetaseThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.• Synthetase will recognize the right tRNA by looking a specific sites along the tRNASpecificity of the threonine synthetase• Threonine and serine have hydroxyl groups that can interact with the zinc• Valine will not interact with the zincDouble-sieve model of editing by aminoacyl-tRNA synthetase• The correct amino acid will be too big to enter the editing site and thus will not be removed• A smaller amino acid will enter the editing site though and will get removed○ Indicates that it was the wrong amino acidThe ribosome has three binding sites for tRNAs• Decoding center is where the mRNA bindsInitiation Site (purine-rich)• UAC  anticodon of initiator tRNA at P site• 16S rRNA -- AUUCCUCCAEF-Tu brings charged tRNA to appropriate codon• Must be recharged with a new GTP molecule○ Which is done by EF-TCovalent linkage of amino acid to cognate tRNA• Ester bond between amino acid and tRNAPeptide bond formation• Amino group of the amino acid is free and will attack the carboxyl


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UIUC MCB 450 - Protein Synthesis

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