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UIUC MCB 450 - Eukaryotic RNA Processing and the Genetic Code

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MCB 450 1st Edition Lecture 24Outline of Last Lecture I. Transcription in prokaryotesa. RNA polymeraseb. Sites of Initiationc. Transcription Elongationd. Transcription terminatione. Transcription Regulation- the lac operonII. Overview of Transcription in EukaryotesOutline of Current Lecture I. Ribosomal RNA processingII. tRNA processingIII. mRNA processingIV. RNA binding proteinsV. The genetic codeVI. Wobble hypothesisVII. tRNA structureVIII. tRNA anticodon binding to codonIX. Aminoacyl-tRNA synthetaseCurrent LectureThe processing of eukaryotic pre-rRNA• Nucleotides are modified first• Then pre-rRNA is cleaved and packaged to form mature ribosomes○ Highly regulated processTransfer RNA precursor processing• Requires the removal of the removal of a 14-nucleotide intron -- yellow• Then the cleavage of the 5' leader -- green• Then the removal of the 3' trailer -- blue• Lastly, the attachment of CCA at the 3' end -- redEukaryotic mRNA Processing• Primary transcript is modified by cap and poly(A) addition• Introns are also removed from the primary transcript to form mRNATranscription and processing of mRNA are coupled • The transcription factor TFIIH phosphorylates the carboxyl-terminal domain of RNA polymerase IIThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.○ This signals the change from transcription initiation to


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UIUC MCB 450 - Eukaryotic RNA Processing and the Genetic Code

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