MCB 450 1st Edition Lecture 24 Outline of Last Lecture I Transcription in prokaryotes a RNA polymerase b Sites of Initiation c Transcription Elongation d Transcription termination e Transcription Regulation the lac operon II Overview of Transcription in Eukaryotes Outline of Current Lecture I Ribosomal RNA processing II tRNA processing III mRNA processing IV RNA binding proteins V The genetic code VI Wobble hypothesis VII tRNA structure VIII tRNA anticodon binding to codon IX Aminoacyl tRNA synthetase Current Lecture The processing of eukaryotic pre rRNA Nucleotides are modified first Then pre rRNA is cleaved and packaged to form mature ribosomes Highly regulated process Transfer RNA precursor processing Requires the removal of the removal of a 14 nucleotide intron yellow Then the cleavage of the 5 leader green Then the removal of the 3 trailer blue Lastly the attachment of CCA at the 3 end red Eukaryotic mRNA Processing Primary transcript is modified by cap and poly A addition Introns are also removed from the primary transcript to form mRNA Transcription and processing of mRNA are coupled The transcription factor TFIIH phosphorylates the carboxyl terminal domain of RNA polymerase II These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute This signals the change from transcription initiation to elongation
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