UNT BIOL 3451 - Recombinant DNA and Technology (5 pages)

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Recombinant DNA and Technology



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Recombinant DNA and Technology

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Looking at genomic libraries, PCR, and other recombinant DNA techniques.


Lecture number:
26
Pages:
5
Type:
Lecture Note
School:
University of North Texas
Course:
Biol 3451 - Genetics
Edition:
1
Documents in this Packet

Unformatted text preview:

BIOL 3451 1st Edition Lecture 26 Outline of Last Lecture I II III 19 8 Environmental Agents Contribute to Human Cancers 20 1 Recombinant DNA Technology Began with Two Key Tools Restriction Enzymes and DNA Cloning Vectors 20 2 DNA Libraries Are Collections of Cloned Sequences Outline of Current Lecture I 20 2 DNA Libraries Are Collections of Cloned Sequences II 20 3 The Polymerase Chain Reaction Is a Powerful Technique for Copying DNA III 20 4 Molecular Techniques for Analyzing DNA IV 20 5 DNA Sequencing Is the Ultimate Way to Characterize DNA Structure at the Molecular Level Current Lecture I 20 2 DNA Libraries Are Collections of Cloned Sequences o A genomic library contains at least one copy of all the sequences in the genome of interest DNA libraries represent a collection of cloned DNA samples derived from a single source that could be a particular tissue type cell type or single individual constructed by cutting genomic DNA with a restriction enzyme and ligating the fragments into vectors which are chosen depending on the size of the genome need to be large to get all of the genome o YACs were commonly used to accommodate large sizes of DNA necessary to span the 3 billion ppb of DNA in the human genome o Whole genome shotgun cloning approaches and next generation sequencing are replacing traditional genomic libraries Today we don t even use cloning techniques to get genome o All DNA fragments in a genomic sample are sequenced without the need to insert DNA fragments into vectors and cloning them in host cells These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II o Complementary DNA cDNA libraries contains complementary DNA copies made from the mRNAs present in a cell population and represents the genes that are transcriptionally active at the time the cells were collected for mRNA isolation Prepared by 1 isolating mRNA from cells take advantage of poly A tail 2



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