UNT BIOL 3451 - Recombinant DNA and Technology (4 pages)

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Recombinant DNA and Technology



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Recombinant DNA and Technology

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A look at the different techniques used in DNA recombination like genomic libraries and how it is useful.


Lecture number:
25
Pages:
4
Type:
Lecture Note
School:
University of North Texas
Course:
Biol 3451 - Genetics
Edition:
1
Documents in this Packet

Unformatted text preview:

BIOL 3451 1st Edition Lecture 25 Outline of Last Lecture I 19 2 Cancer Cells Contain Genetic Defects Affecting Genomic Stability DNA Repair and Chromatin Modifications II 19 3 Cancer Cells Contain Genetic Defects Affecting Cell Cycle Regulation III 19 4 Proto oncogenes and Tumor Suppressor Genes Are Altered in Cancer Cells IV 19 5 Cancer Cells Metastasize and Invade Other Tissues V 19 6 Predisposition to Some Cancers Can Be Inherited VI 19 7 Viruses Contribute to Cancer in Both Humans and Animals VII 19 8 Environmental Agents Contribute to Human Cancers Outline of Current Lecture I 19 8 Environmental Agents Contribute to Human Cancers II 20 1 Recombinant DNA Technology Began with Two Key Tools Restriction Enzymes and DNA Cloning Vectors III 20 2 DNA Libraries Are Collections of Cloned Sequences Current Lecture I 19 8 Environmental Agents Contribute to Human Cancers o Both UVlight and ionizing radiation X rays and gamma rays induce DNA damage Can cause skin cancer Radon gas may be responsible for up to 50 of the ionizing radiation exposure to the US population and contribute to lung cancer II 20 1 Recombinant DNA Technology Began with Two Key Tools Restriction Enzymes and DNA Cloning Vectors o Recombinant DNA joining of DNA molecules usually from different biological sources that are not found together in nature o The basic procedure for producing recombinant DNA generating specific DNA fragments using restriction enzymes joining these fragments with a vector transferring the recombinant DNA molecule to a host cell to produce many copies that can be recovered from the host cell called clones 1 Can be used to study the structure and orientation of the DNA o Recombinant DNA technology is used to isolate replicate and analyze genes o A restriction enzyme binds to DNA at a specific recognition sequence restriction site and cleaves the DNA to produce restriction fragments Figure 20 1 These notes represent a detailed interpretation of the professor s lecture GradeBuddy is



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