AnnouncementsM1D7 Workflow Announcements Lab Quiz Pre‐lab Lecture Today in Lab: M1D7Announcements• HW general comments– Methods: great improvement in judgment of what is essential; slurry vs. resin; specifying concentrations– Introduction: motivate your specific experiment and connect to big picture; need citations• Next time– Meet in 16‐336 at 1:30 sharp for j. club! (Not 1:35 pm.)– You will receive your comments/grades at the meetings with Atissa, beginning next week. (Sign up on Day 8.)M1D7 Workflow1. DNase treatment (30’), prepare spin columns2. Measure [RNA] using spec.3. Dilute and denatureRNAGoal: start by ~ 2:304. Mix RNA with heme; scan Have one partner do all the saving – check 1stwith meCalculate if you have enough to proceed – talk to us if not!A405shiftotherpartnercontrols/benchmarks, too!MIT OpenCourseWarehttp://ocw.mit.edu 20.109 Laboratory Fundamentals in Biological EngineeringSpring 2010 For information about citing these materials or our Terms of Use, visit:
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