Announcements Quiz Pre‐lab Lecture Interpreting transformations E. coli growth Today in Lab (Mod 2 Day 4)Announcements • Report return today, revision due in 2 wks • Some general comments • • Previous FNT #1 careful look @ protocol #2 micro and macro #3 show explicitly Module 2 vs. Module 1 expectationsE. Coli growth curve log density or # cells t=0 Æ dilute cells timeToday in Lab • Obtain DE3 in mid‐log phase, make competent – 1 hour incubation 0.4 –0.6 OD (keep in mind 1:10) • Extract DNA from two mutant candidates • Transform DE3 with the extracted DNA – ½hour incubation • During incubation(s): count mutant colonies, set up diagnostic digests and sequencing rxns – digest 1+ hour, we will stop digests if end past 5 pmMIT OpenCourseWarehttp://ocw.mit.edu 20.109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use, visit:
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