Announcements Quiz Pre lab Lecture Interpreting transformations E coli growth Today in Lab Mod 2 Day 4 Announcements Report return today revision due in 2 wks Some general comments Module 2 vs Module 1 expectations Previous FNT 1 careful look protocol 2 micro and macro 3 show explicitly E Coli growth curve log density or cells t 0 dilute cells time Today in Lab Obtain DE3 in mid log phase make competent 1 hour incubation 0 4 0 6 OD keep in mind 1 10 Extract DNA from two mutant candidates Transform DE3 with the extracted DNA hour incubation During incubation s count mutant colonies set up diagnostic digests and sequencing rxns digest 1 hour we will stop digests if end past 5 pm MIT OpenCourseWare http ocw mit edu 20 109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use visit http ocw mit edu terms
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