Announcements Final Quiz Wrap up comments Announcements Presentations May 11th 12th Starting at 1 35 pm Plan for Thursday May 13th Lecture give feedback then fill out evaluations Afterward lab party at 12 1 30 pm RSVP Final self assessment Optional due to term regs but would be nice to hear your input Can hand in by email by Thursday the 13th ELISA analysis unknowns CN II I ratios also absolute values potentially if latter very small former is meaningless Final assignments etc Report what do I need to know to understand and repeat your experiment given OWW access Experimental plan alginate cells type Amount and quality of RNA RT PCR analysis method ELISA level of replicate agreement Not an exhaustive list Research proposals Rubric is online Assignments page Specify a question and experiments to address it Make clear what is novel aspect s Clean up 4 Optional discussion of data Looking at live cell count vs live cell Understanding transcript assay mechanics What if protein and transcript assays suggest different conclusions low absolute conc differentiated technical reasons degradation rates for I vs II pepsin rates for I vs II biological reasons snapshot vs cumulative processing export optimized for 100ng template ELISA absolute protein RT PCR relative to benchmark each other information 5 MIT OpenCourseWare http ocw mit edu 20 109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use visit http ocw mit edu terms
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