Announcements Final Quiz Wrap‐up commentsAnnouncements • Presentations May 11th/12th – Starting at 1:35 pm • Plan for Thursday, May 13th – Lecture: give feedback, then fill out evaluations – Afterward, lab party at 12 ‐1:30 pm (RSVP) • Final self‐assessment – Optional (due to term regs), but would be nice to hear your input – Can hand in by email, by Thursday the 13thELISA analysis unknowns CN II/I ratios; also absolute values potentially. if latter very small, former is meaningless.Final assignments, etc. • Report: what do I need to know to understand and repeat your experiment, given OWW access? ¾ Experimental plan – alginate, cells (type/#) ¾ Amount and quality of RNA ¾ RT-PCR analysis method ¾ ELISA level of replicate agreement ¾ (Not an exhaustive list!) • Research proposals ¾ Rubric is online (Assignments page) ¾ Specify a question and experiments to address it ¾ Make clear what is novel aspect(s) • Clean-up! 4Optional discussion of data • Looking at live cell count vs. live cell % • Understanding transcript assay mechanics • What if protein and transcript assays suggest different conclusions? low absolute conc.; differentiated � technical reasons degradation rates for I vs. II; � biological reasons pepsin rates for I vs. II; snapshot vs. cumulative; processing/export •optimized for ~100ng template •ELISA – absolute [protein] •RT‐PCR –relative to benchmark/each other information 5MIT OpenCourseWarehttp://ocw.mit.edu 20.109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use, visit:
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