Module 2 Expression Engineering 20 109 Lecture 2 October 16th 2007 Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Public Understanding Image removed due to copyright restrictions From The DNA Diet AKC Gazette April 2006 Scientists are seeking the recipes for dog foods that can attack illness at the genetic level http www ardenmoore com pets dna diet html Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Public Understanding Images removed due to copyright restrictions Website screenshots from http www thednadiet com advertising a service using DNA testing to develop a personal diet plan Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Public Understanding Images removed due to copyright restrictions Photo of anti GMO protesters from http www gmfreeireland org Cartoon of mad scientist character Editorial cartoon about fast track bill to allow use of genetically modified food ingredients without labeling Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Public Understanding Image removed due to copyright restrictions Cartoon of mad scientist character Text removed due to copyright restrictions Excerpt from Lawmakers Administration Clash over Science Unding by Aliya Sternstein National Journal s Technology Daily February 14 2007 http www govexec com story page cfm articleid 36137 ref rellink accessed May 7 2008 Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Expression Engineering Experiment Day 1 Day 2 Day 3 Image of glowing luciferase in a microcentrifuge tube removed due to copyright restrictions RT Figure by MIT OpenCourseWare Day 6 Day 5 Day 4 Image credits Day 1 Courtesy of Arkitek Studios Used with permission Day 2 Courtesy of The Exploratorium The Exploratorium http www exploratorium edu Day 4 Figure by MIT OpenCourseWare Day 5 Dr Natalie Kuldell Day 6 Courtesy of NIH Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY Expression Engineering Experiment Lecture 1 Lecture 2 intro to cell culture transfection intro to gene exp n RNAi luciferase Lecture 3 Lecture 4 off target nonspecific RNAi Writing lecture Neal Lerner Lecture 5 Lecture 6 measuring gene express n microarray analysis Rebecca Fry Lecture 7 Lecture 8 high throughput technologies review of your data or RNAi applications no lab Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move nature s ways Phage infects Occasional phage packages host DNA Hybrid phage infects new cell Host may incorporate foreign DNA Figure by MIT OpenCourseWare Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move nature s ways 1 Viral transduction Text and image removed due to copyright restrictions Excerpt from Pearson Helen Ancient Human Virus Resurrected Nature 31 October 2006 DOI 10 1038 news061030 4 http blogs nature com news blog 2006 10 ancient human virus resurrecte html Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move nature s ways 1 Viral transduction Mobile plasmid Pilus Chromosome A 2 Conjugation Bacterium B Donor Recipient C Relaxosome Transferosome D New donor New donor Figure by MIT OpenCourseWare Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move nature s ways 1 Viral transduction 2 Conjugation 3 Transformation Courtesy Elsevier Inc http www sciencedirect com Used with permission Jonathan M Solomon and Alan D Grossman Who s competent and when regulation of natural genetic competence in bacteria Trends in Genetics 1996 12 4 150 Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move in the lab 1 Viral transduction 2 Conjugation Ca Ca P P P P Figure by MIT OpenCourseWare 3 Transformation chemical electrical Courtesy of Zephyris Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move in the lab 1 Viral transduction Ca Ca 3 Transformation P P P P Figure by MIT OpenCourseWare 2 Conjugation chemical microinjection Image removed due to copyright restrictions electrical Courtesy of Zephyris biolistics Image removed due to copyright restrictions See http firstivf net laboratory tour htm Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu Massachusetts Institute of Technology Downloaded on DD Month YYYY DNA on the move RNAi delivery Courtesy of Markus Stoffel Used with permission Nature Biotechnology 2007 25 1149 1157 Mechanisms and optimization of in vivo delivery of lipophilic siRNAs Cite as Natalie Kuldell Course materials for 20 109 Laboratory Fundamentals in Biological Engineering Fall 2007 MIT OpenCourseWare http ocw mit edu