MIT 20 109 - Basic Statistics;-Standards i Scientific Communities I! (18 pages)

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Basic Statistics;-Standards i Scientific Communities I!



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Basic Statistics;-Standards i Scientific Communities I!

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Pages:
18
School:
Massachusetts Institute of Technology
Course:
20 109 - Laboratory Fundamentals in Biological Engineering

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Basic Statistics Standards in Scienti c Communities I Module 3 Lecture 3 20 109 Spring 2010 Lecture 2 review What properties of hydrogels are advantageous for soft TE What is meant by bioactivity and how can it be introduced What are the two major matrix components of cartilage and how do they support tissue function 2 Topics for Lecture 3 Module 3 so far and Day 3 plan Introduction to statistics con dence intervals t test Standards in scienti c communities general engineering principles standards in synthetic biology standards in data sharing 3 Module progress week 1 Day 1 culture design What did you test BD All rights reserved This content is excluded from our Creative Commons license For more information see http ocw mit edu fairuse Day 2 culture initiation Cells receiving fresh media every 2 days 4 Module day 3 test cell viability Green stain SYTO10 viability Red stain ethidium cytotoxicity Assay readout uorescence Working principle Relative cell permeability 5 Statistics review basics Essential concepts standard deviation s mean sample size n degrees of freedom DOF Normal Gaussian distribution 1 s includes of the data x axis y axis Con dence intervals CI principle 60 sample measured mean 95 CI calculated to be 3 Thus 95 likely that the range 60 3 contains the population true mean exact de nition is subtle 90 CI a where a 3 a 3 a 3 Consider betting example What about n Calculating con dence intervals CI t is tabulated by DOF vs CI DOF n 1 In Excel us TINV function input p value 100 CI 100 Introduction to t test Every statistical test has assumptions asks a speci c question requires human interpretation Some t test assumptions normal distribution cf Mann Whitney test equal variances type 2 in Excel type 3 unequal Question Calculating t test signi cance DOF ttable If tcalc ttable difference is signi cant In Excel us TTEST function Excel returns p value con dence level CL 1 tailed vs 2 tailed test Assignment for report Get live cell count and or live cell percent



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