Announcements Quiz Pre‐lab Lecture Closer Look at Plasmids Site‐Directed Mutagenesis Restriction Enzymes Recap Today in Lab: M2D2antibiotic restae Plasmid Overview plasmids are circular, ds, extrachromosomal isncAmpR ori organism dependent why? vector to introduce a foreign gene (in an organism) Ampicillin resistance Æ select bacteria with Amp that have plasmidSDM Process Dpn1 parent selectively digested due to its methylationToday in Lab • Prepare primer stock and dilution. • SDM rxn: careful not to contaminate shared stocks. • Paper reading period and discussion. • For next time: – Short answer questionsMIT OpenCourseWarehttp://ocw.mit.edu 20.109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use, visit:
View Full Document
Unlocking...