Announcements Quiz Pre lab Lecture Closer Look at Plasmids Site Directed Mutagenesis Restriction Enzymes Recap Today in Lab M2D2 Plasmid Overview an re tib sis io ta tic nc e plasmids are circular ds extrachromosomal AmpR why vector to introduce a foreign gene in an organism Ampicillin resistance select bacteria with Amp that have plasmid ori organism dependent SDM Process Dpn1 parent selectively digested due to its methylation Today in Lab Prepare primer stock and dilution SDM rxn careful not to contaminate shared stocks Paper reading period and discussion For next time Short answer questions MIT OpenCourseWare http ocw mit edu 20 109 Laboratory Fundamentals in Biological Engineering Spring 2010 For information about citing these materials or our Terms of Use visit http ocw mit edu terms
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