Cite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 1 AACGCTACTA CTATTAGTAG AATTGATGCC ACCTTTTCAG CTCGCGCCCC 51 AAATGAAAAT ATAGCTAAAC AGGTTATTGA CCATTTGCGA AATGTATCTA 101 ATGGTCAAAC TAAATCTACT CGTTCGCAGA ATTGGGAATC AACTGTTACA Xba 151 TGGAATGAAA CT[TCTAGA]CA CCGTACTTTA GTTGCATATT TAAAACATGT 201 TGAGCTACAG CACCAGATTC AGCAATTAAG CTCTAAGCCA TCCGCAAAAA 251 TGACCTCTTA TCAAAAGGAG CAATTAAAGG TACTCTCTAA TCCTGACCTG MluI 301 TTGGAGTTTG CTTCCGGTCT GGTTCGCTTT GAAGCTCGAA TTAAA[ACGCG 351 T]TATTTGAAG TCTTTCGGGC TTCCTCTTAA TCTTTTTGAT GCAATCCGCT 401 TTGCTTCTGA CTATAATAGT CAGGGTAAAG ACCTGATTTT TGATTTATGG 451 TCATTCTCGT TTTCTGAACT GTTTAAAGCA TTTGAGGGGG ATTCAATGAA 501 TATTTATGAC GATTCCGCAG TATTGGACGC TATCCAGTCT AAACATTTTA Bsu36I 551 CTATTACCC[C CTCAGG]CAAA ACTTCTTTTG CAAAAGCCTC TCGCTATTTT 601 GGTTTTTATC GTCGTCTGGT AAACGAGGGT TATGATAGTG TTGCTCTTAC 651 TATGCCTCGT AATTCCTTTT GGCGTTATGT ATCTGCATTA GTTGAATGTG MfeI 701 GTATTCCTAA ATCT[CAATTG] ATGAATCTTT CTACCTGTAA TAATGTTGTT 751 CCGTTAGTTC GTTTTATTAA CGTAGATTTT TCTTCCCAAC GTCCTGACTG 801 GTATAATGAG CCAGTTCTTA AAATCGCATA AGGTAATTCA CAATGATTAA 851 AGTTGAAATT AAACCATCTC AAGCCCAATT TACTACTCGT TCTGGTGTTT 901 CTCGTCAGGG CAAGCCTTAT TCACTGAATG AGCAGCTTTG TTACGTTGAT 951 TTGGGTAATG AATATCCGGT TCTTGTCAAG ATTACTCTTG ATGAAGGTCA 1001 GCCAGCCTAT GCGCCTGGTC TGTACACCGT TCATCTGTCC TCTTTCAAAG 1051 TTGGTCAGTT CGGTTCCCTT ATGATTGACC GTCTGCGCCT CGTTCCGGCT 1101 AAGTAACATG GAGCAGGTCG CGGATTTCGA CACAATTTAT CAGGCGATGACite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 1151 TACAAATCTC CGTTGTACTT TGTTTCGCGC TTGGTATAAT CGCTGGGGGT NheI 1201 CAAAGATGAG TGTTTTAGTG TATTCTTTC[G CTAGC]TTCGT TTTAGGTTGG 1251 TGCCTTCGTA GTGGCATTAC GTATTTTACC CGTTTAATGG AAACTTCCTC BglI 1301 ATGAAAAAGT CTTTAGTCCT CAAA[GCCTCT GTGGC]CGTTG CTACCCTCGT 1351 TCCGATGCTG TCTTTCGCTG CTGAGGGTGA CGATCCCGCA AAAGCGGCCT 1401 TTAACTCCCT GCAAGCCTCA GCGACCGAAT ATATCGGTTA TGCGTGGGCG 1451 ATGGTTGTTG TCATTGTCGG CGCAACTATC GGTATCAAGC TGTTTAAGAA 1501 ATTCACCTCG AAAGCAAGCT GATAAACCGA TACAATTAAA GGCTCCTTTT 1551 GGAGCCTTTT TTTTTGGAGA TTTTCAACGT GAAAAAATTA TTATTCGCAA 1601 TTCCTTTAGT TGTTCCTTTC TATTCTCACT CCGCTGAAAC TGTTGAAAGT BlpI 1651 T[GCTTAGC]AA AACCCCATAC AGAAAATTCA TTTACTAACG TCTGGAAAGA 1701 CGACAAAACT TTAGATCGTT ACGCTAACTA TGAGGGTTGT CTGTGGAATG 1751 CTACAGGCGT TGTAGTTTGT ACTGGTGACG AAACTCAGTG TTACGGTACA 1801 TGGGTTCCTA TTGGGCTTGC TATCCCTGAA AATGAGGGTG GTGGCTCTGA 1851 GGGTGGCGGT TCTGAGGGTG GCGGTTCTGA GGGTGGCGGT ACTAAACCTC 1901 CTGAGTACGG TGATACACCT ATTCCGGGCT ATACTTATAT CAACCCTCTC 1951 GACGGCACTT ATCCGCCTGG TACTGAGCAA AACCCCGCTA ATCCTAATCC 2001 TTCTCTTGAG GAGTCTCAGC CTCTTAATAC TTTCATGTTT CAGAATAATA 2051 GGTTCCGAAA TAGGCAGGGG GCATTAACTG TTTATACGGG CACTGTTACT 2101 CAAGGCACTG ACCCCGTTAA AACTTATTAC CAGTACACTC CTGTATCATC 2151 AAAAGCCATG TATGACGCTT ACTGGAACGG TAAATTCAGA GACTGCGCTT 2201 TCCATTCTGG CTTTAATGAG GATCCATTCG TTTGTGAATA TCAAGGCCAA 2251 TCGTCTGACC TGCCTCAACC TCCTGTCAAT GCTGGCGGCG GCTCTGGTGGCite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 2301 TGGTTCTGGT GGCGGCTCTG AGGGTGGTGG CTCTGAGGGT GGCGGTTCTG 2351 AGGGTGGCGG CTCTGAGGGA GGCGGTTCCG GTGGTGGCTC TGGTTCCGGT 2401 GATTTTGATT ATGAAAAGAT GGCAAACGCT AATAAGGGGG CTATGACCGA 2451 AAATGCCGAT GAAAACGCGC TACAGTCTGA CGCTAAAGGC AAACTTGATT 2501 CTGTCGCTAC TGATTACGGT GCTGCTATCG ATGGTTTCAT TGGTGACGTT EcoRI 2551 TCCGGCCTTG CTAATGGTAA TGGTGCTACT GGTGATTTTG CTGGCTC[GAA 2601 TTC]CCAAATG GCTCAAGTCG GTGACGGTGA TAATTCACCT TTAATGAATA 2651 ATTTCCGTCA ATATTTACCT TCCCTCCCTC AATCGGTTGA ATGTCGCCCT 2701 TTTGTCTTTA GCGCTGGTAA ACCATATGAA TTTTCTATTG ATTGTGACAA 2751 AATAAACTTA TTCCGTGGTG TCTTTGCGTT TCTTTTATAT GTTGCCACCT 2801 TTATGTATGT ATTTTCTACG TTTGCTAACA TACTGCGTAA TAAGGAGTCT 2851 TAATCATGCC AGTTCTTTTG GGTATTCCGT TATTATTGCG TTTCCTCGGT 2901 TTCCTTCTGG TAACTTTGTT CGGCTATCTG CTTACTTTTC TTAAAAAGGG 2951 CTTCGGTAAG ATAGCTATTG CTATTTCATT GTTTCTTGCT CTTATTATTG 3001 GGCTTAACTC AATTCTTGTG GGTTATCTCT CTGATATTAG CGCTCAATTA 3051 CCCTCTGACT TTGTTCAGGG TGTTCAGTTA ATTCTCCCGT CTAATGCGCT 3101 TCCCTGTTTT TATGTTATTC TCTCTGTAAA GGCTGCTATT TTCATTTTTG 3151 ACGTTAAACA AAAAATCGTT TCTTATTTGG ATTGGGATAA ATAATATGGC 3201 TGTTTATTTT GTAACTGGCA AATTAGGCTC TGGAAAGACG CTCGTTAGCG 3251 TTGGTAAGAT TCAGGATAAA ATTGTAGCTG GGTGCAAAAT AGCAACTAAT 3301 CTTGATTTAA GGCTTCAAAA CCTCCCGCAA GTCGGGAGGT TCGCTAAAAC 3351 GCCTCGCGTT CTTAGAATAC CGGATAAGCC TTCTATATCT GATTTGCTTG 3401 CTATTGGGCG CGGTAATGAT TCCTACGATG AAAATAAAAA CGGCTTGCTTCite as: Angela Belcher, Drew Endy, Natalie Kuldell and Agi Stachowiak. Course materials for 20.109 Laboratory Fundamentals in Biological Engineering, Fall 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. 3451 GTTCTCGATG AGTGCGGTAC TTGGTTTAAT ACCCGTTCTT GGAATGATAA 3501 GGAAAGACAG CCGATTATTG ATTGGTTTCT ACATGCTCGT AAATTAGGAT 3551 GGGATATTAT TTTTCTTGTT CAGGACTTAT CTATTGTTGA TAAACAGGCG 3601 CGTTCTGCAT TAGCTGAACA TGTTGTTTAT TGTCGTCGTC TGGACAGAAT 3651 TACTTTACCT TTTGTCGGTA CTTTATATTC TCTTATTACT GGCTCGAAAA 3701 TGCCTCTGCC TAAATTACAT GTTGGCGTTG TTAAATATGG CGATTCTCAA 3751 TTAAGCCCTA CTGTTGAGCG TTGGCTTTAT ACTGGTAAGA ATTTGTATAA 3801 CGCATATGAT ACTAAACAGG CTTTTTCTAG TAATTATGAT TCCGGTGTTT 3851 ATTCTTATTT AACGCCTTAT TTATCACACG GTCGGTATTT CAAACCATTA 3901 AATTTAGGTC AGAAGATGAA ATTAACTAAA ATATATTTGA AAAAGTTTTC 3951 TCGCGTTCTT