TAMU BICH 410 - Exam 3 Study Guide (5 pages)

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Exam 3 Study Guide



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Exam 3 Study Guide

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Review of Lecture 18 through 24


Pages:
5
Type:
Study Guide
School:
Texas A&M University
Course:
Bich 410 - Comprehen Biochem I
Edition:
2

Unformatted text preview:

F BICH 410 Study Guide 3 Lectures 18 24 Lectures 18 What are the three types of enzymes and what are they known to do o Oxidoreductase oxidation reduction reaction that typically uses NAD NADH o Lyase breaks molecule and generates double bonds o Ligase brings two molecules together typically ATP or energy source o Isomerase changes the structure of a molecule o Transferase transfers groups o Hydrolase hydrolysis reaction What is the difference between a cofactor coenzyme cosubstrates and prosthetic groups o A cofactor is a small molecule that facilitates a reaction can be a metal ion o A coenzyme is an organic cofactor o A cosubstrate is a coenzyme that transiently binds o A prosthetic group is a coenzyme that permanently binds Which are true and false o Enzymes do not change the free energy TRUE o Enzymes accelerate the rate of the reaction TRUE o Enzymes do not affect the activation energy FALSE o Enzymes effect Keq FALSE o A catalyst lowers the activation barrier equally for both the forward and reverse reaction TRUE What is the equation for the rate enhancement o Rate enhancement e activation energy RT kcat kuncat How do you calculate the free energy o deltaG RTlnKeq keq k1 k2 o deltaG RTln vh kBT Lecture 19 What is the Michaelis Menten Kinetics o E S ES P E How do you calculate specific activity or turnover rate o Specific activity vmax amount of protein o Specific activity vmax amount of protein x molecular weight What is the equation for velocity and how can S and E effect it o Vo Vmax S keq S o If S is significantly less than Km than the eqn is Vo Vmax S keq o If keq S than Vo Vmax 2 o If S is significantly greater than Km than Vo Vmax How do you plot a Lineweaver Burk plot These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute o It is a plot of 1 V versus 1 S o Slope Km Vmax o Km equals the x intercept at 1 km o Vmax equals the y intercept at 1 Vmax What are



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