TAMU BICH 410 - Proteolytic enzymes and Membranes (2 pages)

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Proteolytic enzymes and Membranes



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Proteolytic enzymes and Membranes

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Finishing the enzyme unit and beginning the membrane unit


Lecture number:
23
Pages:
2
Type:
Lecture Note
School:
Texas A&M University
Course:
Bich 410 - Comprehen Biochem I
Edition:
2

Unformatted text preview:

F BICH 410 1st Edition Lecture 23 Outline of Last Lecture Enzymes Chemistry Outline of Current Lecture Proteolytic enzymes hydrolytic enzymes o Aspartic proteases 2 active sites for Asp Kinetic mechanism ping pong Initial velocity plot has parallel lines Chemical mechanism Acid Base catalysis General acid proton donor General base abstracts proton o Initial velocity plot intersecting o HIV Proteases cell translate HIV mrna protein then must be broken down in order for viral growth and cellular infections Drug for AIDS inhibitors of HIV protease o Carboxyl peptidase cleaves of AA residue on C term Arg there to stabilize COO with ionic interactions Zn present indicating metal ionic catalysis Glu is a weak nucleophile therefore C has to be a strong electrophile which is why Zn is used Sequential rxns have all substrates bound and then releases all products Enzyme Regulation o Regulated by availability of S and P rate of synthesis and degradation allosteric rxn covalent modification zymogens isozymes o Zymogen precursor of enzymes proteolytic cleavage converts zymogen to active form Process is irreversible once activated always activated o Isozymes similar but not the same AA seq Each catalyzes same bich rxn but differ in kinetics Different kM and Vmax Ex hexokinase heart and glucokinase liver can have 4 liver subunits or a mix or 4 heart subunits o Allosteric regulation must have multi subunits ie hemoglobin Enzymes that have allosteric regulation sigmoidal Michaelis menton enzymes hyperbolic Allosteric don t obey michaelis menton plot Simplest model T inactive and R active Act as regulatory enzymes in pathways Feedback inhibition product inhibits initial enzymes These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute Feedforward inhibition product actives enzymes in forward rxn o Covalent modifications reversible covalent changes to specific AA side chains Enzymes are used



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