BIOB 375 1st Edition Lecture 33 Outline of Last Lecture II. Gene Amplification III. Cloning vectorOutline of Current Lecture IV. BiotechnologyCurrent LectureBiotechnology- Biotechnology is to use recombinant DNA technology to create commercial products including enzymes, hormones, drugs for medicine, and crops. For medicine:- Pharmaceuticalso Human insulino Human growth hormone- Oligonucleotide drugo Antisense oligonucleotideso Small interfering RNA (siRNA)- Genetic testing- Gene therapy Pharmacuticals:- Human insulin- Human growth hormoneHuman insulin gene in diabetic individuals and human insulin gene in non-diabetic individuals- 1500~3400 bp insertion/deletion of simple sequence repeatsLocated on the short arm of chromosome 11Proinsulin- Insulino A-chaino B-chain- C-peptideBacteria do not have the process to remove intronsThey have a strategy to clone insulin gene without intronsOligonucleotide drug:- Antisense oligonucleotides- Small interfering RNA (siRNA)Using RNAi for the treatment of high cholesterol problemProduction of apoB-siRNA- 21 nt on the sense strand- 23 nt on the anti-sense strandGene therapy- Treatment of human diseases- Somatic gene therapy for immunodeficiency “bobble
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