BIOB 375 1st Edition Lecture 23Outline of Last Lecture I. Antioxidant and redox regulation of gene transcription Outline of Current Lecture II. Regulation of Gene TranscriptionA. Definition of regulon III. Alternative Methods of Gene Regulation at the Transcriptional Level (Quorum Sensing and the Two- Component System)IV. Methods of Gene Regulation at the Translational LevelV. Introduction to MutationsCurrent LectureGenetic Analysis – one gene locus (for organisms that are sexually reproduced)What do we analyzie?1. How many locus is different between the two different individuals with different phenotypes?2. Which phenotype is dominant?3. What is the relationship of one gene locus to a known gene locus (belongs to the same linkage group or not)? One gene locus one characteristic and multiple characteristis- Phiotropic effects on genesOne gene locus can have more than two alleles in the species, but each diploid individual has only two alleles at one locus What do we want to know form genetic analysis?How many locus is responsible for the phenotype?How many alleles at the locus among the individuals?Which phenotypes or allele is dominant, what type of dominance? ( complete, incomplete, or co-dominance)?Genotype predict PhenotypePhenotype deduce GenotypeChlorophyll biosynthesis pathway- 7 enzymes required- 7 genes requiredHow to determine which phenotypes is dominant when two phenotypes are observed?- Make a cross between the two individuals of different phenotypes- If a uniform phenotype observed in F1 generationIf we designate a letter G for the locus that is different between the two P1 and P2 plants, based on the experiment green is dominant, so G represents green and g represents albino When self pollinating, if next generation is heterozygous, the phenotype of that plant is dominant. One gene locus one characteristicA segregating population, in which the individuals were sexually reproduced and have different phenotypes on the characteristic studied, is required for genetic analysisREMEMBER:There are only 4 possible ratios for one gene locusTwo pheotypes when AA and Aa are the same phenotypeAa x Aa 3:1 ratioAa x aa 1:1 ratioUse chi-square test too test if the data fits 3:1
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