BIOB 375 1st Edition Lecture 30Outline of Last Lecture II. Genomicsa. Genetic mapping and physical mappingb. CloningOutline of Current Lecture III. ReviewCurrent LectureREVIEWHow many gene locus is different between the two parents based on the following crosses? Which phenotype is dominant?ParentsShort hair rabbit X short hair rabbitGg x GgOffspring7 short hair, 2 long hair rabbit(Segregating) 3:1 ratioShort hair is dominant, long is recessiveParentsLong hair rabbit X long hair rabbitgg x ggOffspring6 long hair(Not segregating)ParentsLong hair rabbit X short hair rabbitgg x GgOffspring3 short and 2 long hair(Segregating)1:1 ratioWrite the genotype of each parentIf letter G is used for locusWrite genotype of the recessive first ParentsP1(diabetic) X P2(diabetic)aaBB x AAbbOffspring5 normalAaBbParentsP3(normal) X P4(normal)AaBB x AaBBOffspring3 normal, 1 diabetic 3:1 ratioNormal is dominant ParentsP3(normal) X P1(diabetic) AaBB x aaBBOffspring1 normal, 1 diabetic ParentsP4(normal) X P2(diabetic)AaBB x AAbbOffspring5 normalHow do we clone a gene?- Map-based cloning- Homology-based
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