BIOB 375 1st Edition Lecture 10 Outline of Last Lecture I. rRNAa. processing II. TranslationOutline of Current Lecture III. Regulation through changes in chromatin structureA. DNA associationIV. Regulation though changes in chromatin structureV. Gene expression regulated by transcription activatorsVI. Regulation through RNA processingCurrent LectureGene regulation in EukaryotesIn eukaryotes- Genes are not organized into operons. Each structural gene has its own promoter- Zinc fingers and Leucine zipper are common regulatory elements- DNA must unwind form histone proteins before transcriptionRegulation through changes in chromatin structure:- DNA associates with histone to form chromatinRegulation through changes in chromatin structure:- DNase I hypersensitivity- Histone modification- Chromatin remodeling- DNA methylationGene expression regulated by transcription activators, co activators or repressors- Enhancers- Insulators- Coordinated gene regulation- Enhancer 1 can stimulate the transcription of gene A, but its effect on gene B is blocked by the insulator- Enhancer II can stimulate the transcription of gene B, but its effect on gene A is blocked by the insulator- Various proteins may bind to upstream response elements to stimulate transcriptionGene regulation through RNA processing and degradation- Alternative splicing- RNA
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