BIO 326R 1st Edition Lecture 22 Outline of Last Lecture I. Sequencinga. Sanger and Gilbert—Sanger sequencingi. Random sequencingii. Whole genome shot gun sequencingiii. Hierarchical shot gun sequencingb. IllumineII. We have the genome. Now what?a. Sequencing indidualsb. Comparative genomicsc. Personal genome identified by surnamed. sequencing 100s or 1000s of microbial genomes is easyOutline of Current Lecture I. Microbial Group Activities—sociomicrobiologya. Quorum sensing—Vibrio fisherii. Counter illuminationii. Bioluminescenceb. Antivirulent strategiesi. biofilmsCurrent LectureMicrobial Group Activities- Sociomicrobiology- quorum sensingo =count and determine cell densityo Vibrio fisheri= bioluminescent ocean bacteria that lives in symbiosis with squid Bioluminescence= luminescent bacteria Lives on underbelly of the squid and allows the squid to night hunt without a shadow due to the luminescenceThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.- squid hunts at night, sense the light level from the moon, and adjusts a flap on its belly that controls how much v. fisheri are exposed to control the amount of luminescence in order to prevent the presence of a shadowo Counter illumination Light organ increases levels of vibrio fisheri About 10^11 cells/mLo The bacteria use a lot of energy to make light It does not grow when producing light so it will only produce light when the cells are at a certain, high density luxI= homoserine lacto, makes the signal luxR= transcriptional regulator, homoserine lacto binds to luxR and turns on luminescenceo the same system (quorum sensing) in bacteria that makes us sick bacteria control a toxin via quorum sensing it is predicted that inhibiting quarum sensing in these bacteria will not result in resistance because they are not very selective- antivirulent strategieso biofilms= bacteria that generally are attached to surfaces highly resistant to antibiotics- 10 times- 1000 times more antibiotic resistant Surround themselves in a protective polysaccharide layer Attach to surface, micro colonies form (coagulation of bacteria on surface), form mature biofilm community (quarum
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